CDC7/YDL017W Protein Information Help

Standard Name Cdc7p
Systematic Name Ydl017wp
Alias Lsd6p , Sas1p 1
ORF Classification Verified
Description DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p (2, 3, 4, 5, 6)
Name Description Cell Division Cycle 7
Experimental Data
Molecules/cell 1600 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 507
Molecular Weight (Da) 58,320
Isoelectric Point (pI) 5.72

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cdc7p (InterPro)
Physical Interactions There are 96 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012725A | P06243
MIPS: YDL017W
NCBI: 1168818 | 1430983 | 171202 | 3513 | 398364829 | 408137 | 408138 | 683680 | NP_010267.3
GenBank/EMBL/DDBJ: DAA11833.1 | M12624 | X14164 | X15362 | Z48432 | Z74065
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MTSKTKNIDD IPPEIKEEMI QLYHDLPGIE NEYKLIDKIG EGTFSSVYKA
      51  KDITGKITKK FASHFWNYGS NYVALKKIYV TSSPQRIYNE LNLLYIMTGS
     101  SRVAPLCDAK RVRDQVIAVL PYYPHEEFRT FYRDLPIKGI KKYIWELLRA
     151  LKFVHSKGII HRDIKPTNFL FNLELGRGVL VDFGLAEAQM DYKSMISSQN
     201  DYDNYANTNH DGGYSMRNHE QFCPCIMRNQ YSPNSHNQTP PMVTIQNGKV
     251  VHLNNVNGVD LTKGYPKNET RRIKRANRAG TRGFRAPEVL MKCGAQSTKI
     301  DIWSVGVILL SLLGRRFPMF QSLDDADSLL ELCTIFGWKE LRKCAALHGL
     351  GFEASGLIWD KPNGYSNGLK EFVYDLLNKE CTIGTFPEYS VAFETFGFLQ
     401  QELHDRMSIE PQLPDPKTNM DAVDAYELKK YQEEIWSDHY WCFQVLEQCF
     451  EMDPQKRSSA EDLLKTPFFN ELNENTYLLD GESTDEDDVV SSSEADLLDK
     501  DVLLISE*

external links for Cdc7p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cdc7p
1) Axelrod A and Rine J  (1991) A role for CDC7 in repression of transcription at the silent mating-type locus HMR in Saccharomyces cerevisiae. Mol Cell Biol 11(2):1080-91
2) Bell SP and Dutta A  (2002) DNA replication in eukaryotic cells. Annu Rev Biochem 71():333-74
3) Valentin G, et al.  (2006) Dual role of the Cdc7-regulatory protein Dbf4 during yeast meiosis. J Biol Chem 281(5):2828-34
4) Wan L, et al.  (2006) Chemical inactivation of cdc7 kinase in budding yeast results in a reversible arrest that allows efficient cell synchronization prior to meiotic recombination. Genetics 174(4):1767-74
5) Matos J, et al.  (2008) Dbf4-dependent CDC7 kinase links DNA replication to the segregation of homologous chromosomes in meiosis I. Cell 135(4):662-78
6) Lo HC, et al.  (2012) Cdc7-Dbf4 is a gene-specific regulator of meiotic transcription in yeast. Mol Cell Biol 32(2):541-57
7) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41