PTC1/YDL006W Protein Information

Standard Name	Ptc1p 1
Systematic Name	Ydl006wp
Alias	Cwh47p , Kcs2p , Tpd1p
ORF Classification	Verified
Description	Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation (2, 3, 4, 5, 6, 7)
Name Description	Phosphatase type Two C 1

Experimental Data
Molecules/cell	1520 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	281
Molecular Weight (Da)	31,549
Isoelectric Point (pI)	4.72

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ptc1p (InterPro)
Physical Interactions	There are 58 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053134 \| P35182 MIPS: YDL006W NCBI: 1430965 \| 398364893 \| 402503 \| 464326 \| 642800 \| 683691 \| NP_010278.3 GenBank/EMBL/DDBJ: DAA11842.1 \| L14593 \| Z48008 \| Z48432 \| Z74054
External Classifications	EC: 3.1.3.16 [Phosphoprotein phosphatase]

Amino Acid Sequence (or in FASTA format)
1 MSNHSEILER PETPYDITYR VGVAENKNSK FRRTMEDVHT YVKNFASRLD 51 WGYFAVFDGH AGIQASKWCG KHLHTIIEQN ILADETRDVR DVLNDSFLAI 101 DEEINTKLVG NSGCTAAVCV LRWELPDSVS DDSMDLAQHQ RKLYTANVGD 151 SRIVLFRNGN SIRLTYDHKA SDTLEMQRVE QAGGLIMKSR VNGMLAVTRS 201 LGDKFFDSLV VGSPFTTSVE ITSEDKFLIL ACDGLWDVID DQDACELIKD 251 ITEPNEAAKV LVRYALENGT TDNVTVMVVF L*

Amino Acid Sequence (or in FASTA format)

       1  MSNHSEILER PETPYDITYR VGVAENKNSK FRRTMEDVHT YVKNFASRLD
      51  WGYFAVFDGH AGIQASKWCG KHLHTIIEQN ILADETRDVR DVLNDSFLAI
     101  DEEINTKLVG NSGCTAAVCV LRWELPDSVS DDSMDLAQHQ RKLYTANVGD
     151  SRIVLFRNGN SIRLTYDHKA SDTLEMQRVE QAGGLIMKSR VNGMLAVTRS
     201  LGDKFFDSLV VGSPFTTSVE ITSEDKFLIL ACDGLWDVID DQDACELIKD
     251  ITEPNEAAKV LVRYALENGT TDNVTVMVVF L*

external links for Ptc1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ptc1p

1)	Maeda T, et al. (1993) Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol 13(9):5408-17
2)	Robinson MK, et al. (1994) TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation. Mol Cell Biol 14(6):3634-45
3)	van Zyl WH, et al. (1989) A general screen for mutant of Saccharomyces cerevisiae deficient in tRNA biosynthesis. Genetics 123(1):55-68
4)	Maeda T, et al. (1994) A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369(6477):242-5
5)	Roeder AD, et al. (1998) Mitochondrial inheritance is delayed in Saccharomyces cerevisiae cells lacking the serine/threonine phosphatase PTC1. Mol Biol Cell 9(4):917-30
6)	Warmka J, et al. (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51-60
7)	Malleshaiah MK, et al. (2010) The scaffold protein Ste5 directly controls a switch-like mating decision in yeast. Nature 465(7294):101-5
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41