MED2/YDL005C Protein Information

Standard Name	Med2p 1
Systematic Name	Ydl005cp
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia (1, 2, 3)
Name Description	MEDiator complex 1

Experimental Data
Molecules/cell	10800 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	431
Molecular Weight (Da)	47,717
Isoelectric Point (pI)	4.2

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Med2p (InterPro)
Physical Interactions	There are 112 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053152 \| Q12124 MIPS: YDL005C NCBI: 1430963 \| 398364901 \| 45270202 \| 642801 \| 683692 \| 74583810 \| NP_010279.3 \| NM_001180064.3 GenBank/EMBL/DDBJ: DAA11843.1 \| AY558156 \| Z48008 \| Z48432 \| Z74053

Amino Acid Sequence (or in FASTA format)
1 MVVQNSPVSS VHTANFSERG SNTRTMTYKN KLTVCFDDIL KVGAEMMMQQ 51 QLKNVQLDSY LVNGFSQSQQ KLLKEKVKLF HGILDDLETS LSQSSSYLET 101 LTALGKEKEK EREEAEKKRA EQENMRKVRE QEELKKRQEL EEASQQQQLQ 151 QNSKEKNGLG LNFSTTAPAN TTDANGSKEN YQELGSLQSS SQTQLENANA 201 ANNGAAFSPL TTTRIQSQQA QPSDVMFNDL NSMDISMFSG LDSTGFDSTA 251 FNATVDETKG FDDNDSGNNY NDINISSIEN NINNNINSTK NGKDNNNESN 301 KNNNGDEKNK NNNEDNENNN NSSEKNNNNN NNNNNNNDDN GNNNNNNSGN 351 DNNNTTNNDS NNKNNSITTG NDNENIVNND LPTTVVSNPG DNPPPADNGE 401 EYLTLNDFND LNIDWSTTGD NGELDLSGFN I*

Amino Acid Sequence (or in FASTA format)

       1  MVVQNSPVSS VHTANFSERG SNTRTMTYKN KLTVCFDDIL KVGAEMMMQQ
      51  QLKNVQLDSY LVNGFSQSQQ KLLKEKVKLF HGILDDLETS LSQSSSYLET
     101  LTALGKEKEK EREEAEKKRA EQENMRKVRE QEELKKRQEL EEASQQQQLQ
     151  QNSKEKNGLG LNFSTTAPAN TTDANGSKEN YQELGSLQSS SQTQLENANA
     201  ANNGAAFSPL TTTRIQSQQA QPSDVMFNDL NSMDISMFSG LDSTGFDSTA
     251  FNATVDETKG FDDNDSGNNY NDINISSIEN NINNNINSTK NGKDNNNESN
     301  KNNNGDEKNK NNNEDNENNN NSSEKNNNNN NNNNNNNDDN GNNNNNNSGN
     351  DNNNTTNNDS NNKNNSITTG NDNENIVNND LPTTVVSNPG DNPPPADNGE
     401  EYLTLNDFND LNIDWSTTGD NGELDLSGFN I*

external links for Med2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Med2p

1)	Myers LC, et al. (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
3)	Ghosh Dastidar R, et al. (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41