MCD1/YDL003W Protein Information

Standard Name	Mcd1p 1
Systematic Name	Ydl003wp
Alias	Pds3p , Rhc21p 2 , Scc1p 3
ORF Classification	Verified
Description	Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase (1, 4)
Name Description	Mitotic Chromosome Determinant 1

Experimental Data
Molecules/cell	1040 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	566
Molecular Weight (Da)	63,290
Isoelectric Point (pI)	4.35

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mcd1p (InterPro)
Physical Interactions	There are 94 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000530AF \| Q12158 MIPS: YDL003W NCBI: 1430959 \| 2570096 \| 30580504 \| 55670519 \| 55670520 \| 55670521 \| 55670522 \| 6320201 \| 642803 \| 683694 \| 777398 \| NP_010281.1 GenBank/EMBL/DDBJ: DAA11845.1 \| U23759 \| Y14280 \| Z48008 \| Z48432 \| Z74051

Amino Acid Sequence (or in FASTA format)
1 MVTENPQRLT VLRLATNKGP LAQIWLASNM SNIPRGSVIQ THIAESAKEI 51 AKASGCDDES GDNEYITLRT SGELLQGIVR VYSKQATFLL TDIKDTLTKI 101 SMLFKTSQKM TSTVNRLNTV TRVHQLMLED AVTEREVLVT PGLEFLDDTT 151 IPVGLMAQEN SMERKVQGAA PWDTSLEVGR RFSPDEDFEH NNLSSMNLDF 201 DIEEGPITSK SWEEGTRQSS RNFDTHENYI QDDDFPLDDA GTIGWDLGIT 251 EKNDQNNDDD DNSVEQGRRL GESIMSEEPT DFGFDLDIEK EAPAGNIDTI 301 TDAMTESQPK QTGTRRNSKL LNTKSIQIDE ETENSESIAS SNTYKEERSN 351 NLLTPQPTNF TTKRLWSEIT ESMSYLPDPI LKNFLSYESL KKRKIHNGRE 401 GSIEEPELNV SLNLTDDVIS NAGTNDNSFN ELTDNMSDFV PIDAGLNEAP 451 FPEENIIDAK TRNEQTTIQT EKVRPTPGEV ASKAIVQMAK ILRKELSEEK 501 EVIFTDVLKS QANTEPENIT KREASRGFFD ILSLATEGCI GLSQTEAFGN 551 IKIDAKPALF ERFINA*

Amino Acid Sequence (or in FASTA format)

       1  MVTENPQRLT VLRLATNKGP LAQIWLASNM SNIPRGSVIQ THIAESAKEI
      51  AKASGCDDES GDNEYITLRT SGELLQGIVR VYSKQATFLL TDIKDTLTKI
     101  SMLFKTSQKM TSTVNRLNTV TRVHQLMLED AVTEREVLVT PGLEFLDDTT
     151  IPVGLMAQEN SMERKVQGAA PWDTSLEVGR RFSPDEDFEH NNLSSMNLDF
     201  DIEEGPITSK SWEEGTRQSS RNFDTHENYI QDDDFPLDDA GTIGWDLGIT
     251  EKNDQNNDDD DNSVEQGRRL GESIMSEEPT DFGFDLDIEK EAPAGNIDTI
     301  TDAMTESQPK QTGTRRNSKL LNTKSIQIDE ETENSESIAS SNTYKEERSN
     351  NLLTPQPTNF TTKRLWSEIT ESMSYLPDPI LKNFLSYESL KKRKIHNGRE
     401  GSIEEPELNV SLNLTDDVIS NAGTNDNSFN ELTDNMSDFV PIDAGLNEAP
     451  FPEENIIDAK TRNEQTTIQT EKVRPTPGEV ASKAIVQMAK ILRKELSEEK
     501  EVIFTDVLKS QANTEPENIT KREASRGFFD ILSLATEGCI GLSQTEAFGN
     551  IKIDAKPALF ERFINA*

external links for Mcd1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Mcd1p

1)	Guacci V, et al. (1997) A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae. Cell 91(1):47-57
2)	Heo SJ, et al. (1998) The RHC21 gene of budding yeast, a homologue of the fission yeast rad21+ gene, is essential for chromosome segregation. Mol Gen Genet 257(2):149-56
3)	Michaelis C, et al. (1997) Cohesins: chromosomal proteins that prevent premature separation of sister chromatids. Cell 91(1):35-45
4)	Yang H, et al. (2008) Cleavage of Mcd1 by Caspase-like Protease Esp1 Promotes Apoptosis in Budding Yeast. Mol Biol Cell 19(5):2127-34
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41