PTC6/YCR079W Protein Information

Standard Name	Ptc6p 1
Systematic Name	Ycr079wp
Alias	Aup1p 2 , Ppp2p 3
ORF Classification	Verified
Description	Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase (1, 2, 3, 4, 5)
Name Description	Phosphatase Two C 1

Experimental Data
Molecules/cell	432 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	442
Molecular Weight (Da)	49,420
Isoelectric Point (pI)	7

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ptc6p (InterPro)
Physical Interactions	There are 29 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005329A \| P25646 MIPS: YCR079W NCBI: 10383808 \| 14588958 \| 49066025 \| NP_010003.2 GenBank/EMBL/DDBJ: DAA07550.1 \| X59720
External Classifications	EC: 3.1.3.16 [Phosphoprotein phosphatase]

Amino Acid Sequence (or in FASTA format)
1 MRLGNAYAYC KPSQNVGLKL DLLRGLPGYV GHATSRINRL ENQDNYSIKM 51 MRSWPNAYGS ALNCSVFDGH GEKGAQLSQL LADKLCSSLD FPEPSWDKQD 101 LKKLVQEYAR RFPEGNYWKH KLSTFEKFYN KFIKNCNSKQ ELLLMKEGDS 151 AILGQNGGRM IFDKMGNIID KIALLTELDR LRLFYGFARF DLDQCCGLGT 201 AAGSTASSIF LYPYDDPNAP IDEGKDDDSW IISHSGLLKL IVTQVGDSKI 251 ILCDQDGIAH ALTTTHHINS SRERHRLSID PSRLDPDAFG ETRFLNNFAN 301 TRSFGDVAGK PYGISSEPDI FSFLVGNTLH LPRSERSKLP FNGDECFLAL 351 VTDGITNKLA DQEVVDLITS TVNSWGLKKA TPQFVAEETI KFIQAIATKH 401 SDNATCVVVR LSNWGNWPNV DRTGPQRETK LMNAQSNETK LN*

Amino Acid Sequence (or in FASTA format)

       1  MRLGNAYAYC KPSQNVGLKL DLLRGLPGYV GHATSRINRL ENQDNYSIKM
      51  MRSWPNAYGS ALNCSVFDGH GEKGAQLSQL LADKLCSSLD FPEPSWDKQD
     101  LKKLVQEYAR RFPEGNYWKH KLSTFEKFYN KFIKNCNSKQ ELLLMKEGDS
     151  AILGQNGGRM IFDKMGNIID KIALLTELDR LRLFYGFARF DLDQCCGLGT
     201  AAGSTASSIF LYPYDDPNAP IDEGKDDDSW IISHSGLLKL IVTQVGDSKI
     251  ILCDQDGIAH ALTTTHHINS SRERHRLSID PSRLDPDAFG ETRFLNNFAN
     301  TRSFGDVAGK PYGISSEPDI FSFLVGNTLH LPRSERSKLP FNGDECFLAL
     351  VTDGITNKLA DQEVVDLITS TVNSWGLKKA TPQFVAEETI KFIQAIATKH
     401  SDNATCVVVR LSNWGNWPNV DRTGPQRETK LMNAQSNETK LN*

external links for Ptc6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ptc6p

1)	Ruan H, et al. (2007) The YCR079w gene confers a rapamycin-resistant function and encodes the sixth type 2C protein phosphatase in Saccharomyces cerevisiae. FEMS Yeast Res 7(2):209-15
2)	Tal R, et al. (2007) Aup1p, a yeast mitochondrial protein phosphatase homolog, is required for efficient stationary phase mitophagy and cell survival. J Biol Chem 282(8):5617-24
3)	Gey U, et al. (2008) Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases. J Biol Chem 283(15):9759-67
4)	Tomaska L (2000) Mitochondrial protein phosphorylation: lessons from yeasts. Gene 255(1):59-64
5)	Sakumoto N, et al. (2002) A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysis. Yeast 19(7):587-99
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41