PRE4/YFR050C Protein Information

Standard Name	Pre4p 1
Systematic Name	Yfr050cp
ORF Classification	Verified
Description	Beta 7 subunit of the 20S proteasome (2, 3)
Name Description	PRoteinase yscE 1

Experimental Data
Molecules/cell	16900 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	266
Molecular Weight (Da)	29,443
Isoelectric Point (pI)	5.81

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pre4p (InterPro)
Physical Interactions	There are 82 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FDD \| P30657 MIPS: YFR050C NCBI: 112490855 \| 112490869 \| 11513425 \| 11513439 \| 11514004 \| 11514018 \| 14318575 \| 14488818 \| 178847472 \| 178847486 \| 178847514 \| 178847528 \| 190016379 \| 190016393 \| 197725333 \| 197725347 \| 20150389 \| 20150396 \| 211939224 \| 211939238 \| 211939252 \| 211939266 \| 238828249 \| 238828263 \| 258588333 \| 258588347 \| 258588361 \| 258588375 \| 258588555 \| 258588569 \| 266838 \| 298508235 \| 298508249 \| 298508451 \| 298508465 \| 3114282 \| 3114296 \| 323462948 \| 323462962 \| 323462976 \| 323462990 \| 323463004 \| 323463018 \| 330689393 \| 330689407 \| 333361156 \| 333361170 \| 333361184 \| 333361198 \| 333361212 \| 333361226 \| 333361240 \| 333361254 \| 335892353 \| 335892367 \| 340780431 \| 340780445 \| 340780459 \| 340780473 \| 350610684 \| 350610698 \| 359545858 \| 359545872 \| 378792253 \| 378792287 \| 390980828 \| 390980842 \| 390980856 \| 390980870 \| 402550744 \| 403071960 \| 403072204 \| 409973872 \| 409973900 \| 4223 \| 444302351 \| 444302379 \| 444302407 \| 51013345 \| 75765712 \| 836805 \| 93279380 \| 93279394 \| 99032214 \| 99032228 \| NP_116708.1 GenBank/EMBL/DDBJ: DAA12493.1 \| AY692947 \| D50617 \| X68663
External Classifications	EC: 3.4.25.1 [Proteasome endopeptidase complex]

Amino Acid Sequence (or in FASTA format)
1 MNHDPFSWGR PADSTYGAYN TQIANAGASP MVNTQQPIVT GTSVISMKYD 51 NGVIIAADNL GSYGSLLRFN GVERLIPVGD NTVVGISGDI SDMQHIERLL 101 KDLVTENAYD NPLADAEEAL EPSYIFEYLA TVMYQRRSKM NPLWNAIIVA 151 GVQSNGDQFL RYVNLLGVTY SSPTLATGFG AHMANPLLRK VVDRESDIPK 201 TTVQVAEEAI VNAMRVLYYR DARSSRNFSL AIIDKNTGLT FKKNLQVENM 251 KWDFAKDIKG YGTQKI*

Amino Acid Sequence (or in FASTA format)

       1  MNHDPFSWGR PADSTYGAYN TQIANAGASP MVNTQQPIVT GTSVISMKYD
      51  NGVIIAADNL GSYGSLLRFN GVERLIPVGD NTVVGISGDI SDMQHIERLL
     101  KDLVTENAYD NPLADAEEAL EPSYIFEYLA TVMYQRRSKM NPLWNAIIVA
     151  GVQSNGDQFL RYVNLLGVTY SSPTLATGFG AHMANPLLRK VVDRESDIPK
     201  TTVQVAEEAI VNAMRVLYYR DARSSRNFSL AIIDKNTGLT FKKNLQVENM
     251  KWDFAKDIKG YGTQKI*

external links for Pre4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pre4p

1)	Hilt W, et al. (1993) The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis. J Biol Chem 268(5):3479-86
2)	Groll M, et al. (1999) The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study. Proc Natl Acad Sci U S A 96(20):10976-83
3)	Jager S, et al. (1999) Proteasome beta-type subunits: unequal roles of propeptides in core particle maturation and a hierarchy of active site function. J Mol Biol 291(4):997-1013
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41