DUG1/YFR044C Protein Information

Standard Name	Dug1p 1
Systematic Name	Yfr044cp
ORF Classification	Verified
Description	Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) (1, 2)
Name Description	Deficient in Utilization of Glutathione 1

Experimental Data
Molecules/cell	22300 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	481
Molecular Weight (Da)	52,871
Isoelectric Point (pI)	5.43

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dug1p (InterPro)
Physical Interactions	There are 15 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F2D \| P43616 MIPS: YFR044C NCBI: 1176021 \| 14318569 \| 51012855 \| 836799 \| NP_116702.1 GenBank/EMBL/DDBJ: DAA12487.1 \| AY692702 \| D50617

Amino Acid Sequence (or in FASTA format)
1 MSHSLTSVFQ KIDSLKPQFF SRLTKAIQIP AVSSDESLRS KVFDKAKFIS 51 EQLSQSGFHD IKMVDLGIQP PPISTPNLSL PPVILSRFGS DPSKKTVLVY 101 GHYDVQPAQL EDGWDTEPFK LVIDEAKGIM KGRGVTDDTG PLLSWINVVD 151 AFKASGQEFP VNLVTCFEGM EESGSLKLDE LIKKEANGYF KGVDAVCISD 201 NYWLGTKKPV LTYGLRGCNY YQTIIEGPSA DLHSGIFGGV VAEPMIDLMQ 251 VLGSLVDSKG KILIDGIDEM VAPLTEKEKA LYKDIEFSVE ELNAATGSKT 301 SLYDKKEDIL MHRWRYPSLS IHGVEGAFSA QGAKTVIPAK VFGKFSIRTV 351 PDMDSEKLTS LVQKHCDAKF KSLNSPNKCR TELIHDGAYW VSDPFNAQFT 401 AAKKATKLVY GVDPDFTREG GSIPITLTFQ DALNTSVLLL PMGRGDDGAH 451 SINEKLDISN FVGGMKTMAA YLQYYSESPE N*

Amino Acid Sequence (or in FASTA format)

       1  MSHSLTSVFQ KIDSLKPQFF SRLTKAIQIP AVSSDESLRS KVFDKAKFIS
      51  EQLSQSGFHD IKMVDLGIQP PPISTPNLSL PPVILSRFGS DPSKKTVLVY
     101  GHYDVQPAQL EDGWDTEPFK LVIDEAKGIM KGRGVTDDTG PLLSWINVVD
     151  AFKASGQEFP VNLVTCFEGM EESGSLKLDE LIKKEANGYF KGVDAVCISD
     201  NYWLGTKKPV LTYGLRGCNY YQTIIEGPSA DLHSGIFGGV VAEPMIDLMQ
     251  VLGSLVDSKG KILIDGIDEM VAPLTEKEKA LYKDIEFSVE ELNAATGSKT
     301  SLYDKKEDIL MHRWRYPSLS IHGVEGAFSA QGAKTVIPAK VFGKFSIRTV
     351  PDMDSEKLTS LVQKHCDAKF KSLNSPNKCR TELIHDGAYW VSDPFNAQFT
     401  AAKKATKLVY GVDPDFTREG GSIPITLTFQ DALNTSVLLL PMGRGDDGAH
     451  SINEKLDISN FVGGMKTMAA YLQYYSESPE N*

external links for Dug1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Dug1p

1)	Ganguli D, et al. (2007) The Alternative Pathway of Glutathione Degradation Is Mediated by a Novel Protein Complex Involving Three New Genes in Saccharomyces cerevisiae. Genetics 175(3):1137-51
2)	Kaur H, et al. (2009) Dug1p Is a Cys-Gly Peptidase of the {gamma}-Glutamyl Cycle of Saccharomyces cerevisiae and Represents a Novel Family of Cys-Gly Peptidases. J Biol Chem 284(21):14493-502
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41