CDC14/YFR028C Protein Information Help

Standard Name Cdc14p 1
Systematic Name Yfr028cp
Alias Oaf3p
ORF Classification Verified
Description Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis (2, 3, 4, 5, 6, 7)
Name Description Cell Division Cycle 8
Experimental Data
Molecules/cell 8550 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 551
Molecular Weight (Da) 61,906
Isoelectric Point (pI) 7.99

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cdc14p (InterPro)
Physical Interactions There are 275 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000004DC1A | Q00684
MIPS: YFR028C
NCBI: 1168807 | 171183 | 398364341 | 407518 | 836783 | 870756 | NP_116684.3 | NM_001179993.3
GenBank/EMBL/DDBJ: DAA12468.1 | D50617 | D55715 | M61194 | X75077
External Classifications EC: 3.1.3.48 [Protein-tyrosine-phosphatase]
Amino Acid Sequence (or in FASTA format)
 
       1  MRRSVYLDNT IEFLRGRVYL GAYDYTPEDT DELVFFTVED AIFYNSFHLD
      51  FGPMNIGHLY RFAVIFHEIL NDPENANKAV VFYSSASTRQ RANAACMLCC
     101  YMILVQAWTP HQVLQPLAQV DPPFMPFRDA GYSNADFEIT IQDVVYGVWR
     151  AKEKGLIDLH SFNLESYEKY EHVEFGDFNV LTPDFIAFAS PQEDHPKGYL
     201  ATKSSHLNQP FKSVLNFFAN NNVQLVVRLN SHLYNKKHFE DIGIQHLDLI
     251  FEDGTCPDLS IVKNFVGAAE TIIKRGGKIA VHCKAGLGRT GCLIGAHLIY
     301  TYGFTANECI GFLRFIRPGM VVGPQQHWLY LHQNDFREWK YTTRISLKPS
     351  EAIGGLYPLI SLEEYRLQKK KLKDDKRVAQ NNIEGELRDL TMTPPSNGHG
     401  ALSARNSSQP STANNGSNSF KSSAVPQTSP GQPRKGQNGS NTIEDINNNR
     451  NPTSHANRKV VIESNNSDDE SMQDTNGTSN HYPKVSRKKN DISSASSSRM
     501  EDNEPSATNI NNAADDTILR QLLPKNRRVT SGRRTTSAAG GIRKISGSIK
     551  K*

external links for Cdc14p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Cdc14p
1) Culotti J and Hartwell LH  (1971) Genetic control of the cell division cycle in yeast. 3. Seven genes controlling nuclear division. Exp Cell Res 67(2):389-401
2) Wan J, et al.  (1992) CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle. J Biol Chem 267(16):11274-80
3) Visintin R, et al.  (1998) The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation. Mol Cell 2(6):709-18
4) Taylor GS, et al.  (1997) The activity of Cdc14p, an oligomeric dual specificity protein phosphatase from Saccharomyces cerevisiae, is required for cell cycle progression. J Biol Chem 272(38):24054-63
5) Saunders WS  (2002) The FEAR factor. Mol Cell 9(2):207-9
6) Marston AL, et al.  (2003) The Cdc14 phosphatase and the FEAR network control meiotic spindle disassembly and chromosome segregation. Dev Cell 4(5):711-26
7) Bizzari F and Marston AL  (2011) Cdc55 coordinates spindle assembly and chromosome disjunction during meiosis. J Cell Biol 193(7):1213-28
8) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41