ATG18/YFR021W Protein Information Help

Standard Name Atg18p 1
Systematic Name Yfr021wp
Alias Nmr1p 2 , Cvt18p 3 , Aut10p 4 , Svp1p 5
ORF Classification Verified
Description Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood (4, 5, 6, 7, 8)
Name Description AuTophaGy related 1
Experimental Data
Molecules/cell 1560 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 500
Molecular Weight (Da) 55,102
Isoelectric Point (pI) 5.28

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Atg18p (InterPro)
Physical Interactions There are 45 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005328A | P43601
MIPS: YFR021W
NCBI: 1176002 | 16740527 | 836776 | NP_444297.1 | NM_001179986.1
GenBank/EMBL/DDBJ: DAA12461.1 | D50617
Amino Acid Sequence (or in FASTA format)
 
       1  MSDSSPTINF INFNQTGTCI SLGTSKGFKI FNCEPFGKFY SEDSGGYAIV
      51  EMLFSTSLLA LVGIGDQPAL SPRRLRIINT KKHSIICEVT FPTSILSVKM
     101  NKSRLVVLLQ EQIYIYDINT MRLLHTIETN PNPRGLMAMS PSVANSYLVY
     151  PSPPKVINSE IKAHATTNNI TLSVGGNTET SFKRDQQDAG HSDISDLDQY
     201  SSFTKRDDAD PTSSNGGNSS IIKNGDVIVF NLETLQPTMV IEAHKGEIAA
     251  MAISFDGTLM ATASDKGTII RVFDIETGDK IYQFRRGTYA TRIYSISFSE
     301  DSQYLAVTGS SKTVHIFKLG HSMSNNKLDS DDSNMEEAAA DDSSLDTTSI
     351  DALSDEENPT RLAREPYVDA SRKTMGRMIR YSSQKLSRRA ARTLGQIFPI
     401  KVTSLLESSR HFASLKLPVE TNSHVMTISS IGSPIDIDTS EYPELFETGN
     451  SASTESYHEP VMKMVPIRVV SSDGYLYNFV MDPERGGDCL ILSQYSILMD
     501  *

external links for Atg18p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Atg18p
1) Klionsky DJ, et al.  (2003) A unified nomenclature for yeast autophagy-related genes. Dev Cell 5(4):539-45
2) Rabitsch KP, et al.  (2001) A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol 11(13):1001-9
3) Guan J, et al.  (2001) Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris. Mol Biol Cell 12(12):3821-38
4) Barth H, et al.  (2001) Autophagy and the cytoplasm to vacuole targeting pathway both require Aut10p. FEBS Lett 508(1):23-8
5) Dove SK, et al.  (2004) Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors. EMBO J 23(9):1922-33
6) Reggiori F, et al.  (2004) The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure. Dev Cell 6(1):79-90
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Saitsu H, et al.  (2013) De novo mutations in the autophagy gene WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood. Nat Genet 45(4):445-9, 449e1
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41