FAB1/YFR019W Protein Information

Standard Name	Fab1p 1
Systematic Name	Yfr019wp
Alias	Svl7p 2
ORF Classification	Verified
Description	1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis (3)
Name Description	Forms Aploid and Binucleate cells 1

Experimental Data
Molecules/cell	149 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	2,278
Molecular Weight (Da)	257,419
Isoelectric Point (pI)	8.56

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fab1p (InterPro)
Physical Interactions	There are 36 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012A42C \| P34756 MIPS: YFR019W NCBI: 330443539 \| 347595800 \| 398498 \| 462047 \| 836774 \| NP_116674.2 GenBank/EMBL/DDBJ: DAA12460.2 \| D50617 \| U01017
External Classifications	EC: 2.7.1.150 [1-phosphatidylinositol-3-phosphate 5-kinase]

Amino Acid Sequence (or in FASTA format)
1 MSSEEPHASI SFPDGSHVRS SSTGTSSVNT IDATLSRPNY IKKPSLHIMS 51 TSTTSTTTDL VTNPILSNIS VPKISPPTSS SIATATSTSH VTGTASHSNI 101 KANANTSTSV NKKNLPPTTS GRIPSSTIKR YPSRYKPSHS LQLPIKNDSN 151 FKRSSIYASK STVTAIPIRN NRPISMQNSY ARTPDSDHDD VGDEVSSIKS 201 ASSSLTASLS KSFLFAFYNN RKKDKTSNNG VLSKEYWMKD ESSKECFSCG 251 KTFNTFRRKH HCRICGQIFC SSCTLLIDGD RFGCHAKMRV CYNCYEHADT 301 YEDSSDEEND STMQLNEPRS RSRSRSSNTN PYSHSHSHLH LISQDNHNGT 351 DLHDPVAATD NPQQQNEVYL LNDDDVQSIM TSGEDSKLFI STPPPPPKMA 401 IPATKQGGSL EISFDSENDR ALHYQDDNPG RHHHLDSVPT RYTIRDMDNI 451 SHYDTNSNST LRPHYNTNNS TITINNLNNT TSNNSNYNNT NSNSNINNPA 501 HSLRRSIFHY VSSNSVNKDS NNSSATPASS AQSSSILDPA NRIIGNYAHR 551 NYKFKFNYNS KGPSQQNDTA NGNNDNNNNN NNNNNNNNNN SASGIADNNN 601 IPSNDNGTTF TLDKKKRNPL TKSKSTSAYL EYPLNEEDSS EDEGSMSIYS 651 VLNDDHKTDN PIRSMRNSTK SFQRAQASLQ RMRFRRKSKS KHFPNNSKSS 701 IYRDLNFLTN STPNLLSVVS DDNLYDDSSP LQDKASSSAA SRLTDRKFSN 751 SSGSNNNSNS NSNINTDPWK RIASISGFKL KKEKKRELNE VSLLHMHALL 801 KQLLNDQEIS NLQEWITLLD GALRKVLRTI LNARDLNTLD FRQTYVKIKR 851 ISGGSPQNSE YIDGVVFSKA LPSKTMPRHL KNPRILLIMF PLEYQKNNNH 901 FLSIESVFRQ EREYLDKLVS RLKSLHPDII YVGANVSGYA LELLNDSGIV 951 VQFNMKPQVI ERIAKLTEAD IAISVDKLAT NIKMGECETF EVKSYIYGNI 1001 SKTYTFLRGC NPELGGTILL RGDSLENLRK IKQVSEFMVY AIFSLKLESS 1051 FFNDNFIQLS TDVYLKRAES KKLQVFEGYF ADFLIKFNNR ILTVSPTVDF 1101 PIPFLLEKAR GLEKKLIERI NQYESESDLD RQTQLNMLQG LESTITKKHL 1151 GNLIKFLHEM EIENLELEFQ KRSRQWEVSY SSSQNLLGTG SHQSITVLYS 1201 MVSTKTATPC VGPQIVTIDY FWDSDISIGQ FIENVVGTAR YPCQQGCNGL 1251 YLDHYRSYVH GSGKVDVLIE KFQTRLPKLK DIILTWSYCK KCGTSTPILQ 1301 ISEKTWNHSF GKYLEVMFWS YKDSVTGIGK CPHDFTKDHV KYFGYNDLVV 1351 RLEYSDLEVH ELITPPRKIK WKPHIDIKLK VELYYKILEK INNFYGSVLS 1401 RLERIKLDSM TKDKVLSGQA KIIELKSNAT EEQKLMLQDL DTFYADSPCD 1451 QHLPLNLVIK SLYDKAVNWN STFAIFAKSY LPSETDISRI TAKQLKKLFY 1501 DSSRKDSEDK KSLHDEKAKT RKPEKNELPL EGLKDVEKPK IDSKNTTENR 1551 DRTNEPQNAV TITTFKDDTP IIPTSGTSHL TVTPSASSVS SSLTPQTEER 1601 PPISRSGTGI SMTHDKSTRP NIRKMSSDSS LCGLASLANE YSKNNKVSKL 1651 ATFFDQMHFD ALSKEFELER ERERLQLNKD KYQAIRLQTS TPIVEIYKNV 1701 KDAVDEPLHS RSSGNNLSSA NVKTLEAPVG EHSRANNCNP PNLDQNLETE 1751 LENSISQWGE NILNPSGKTT ASTHLNSKPV VKETSENPKS IVRESDNSKS 1801 EPLPPVITTT TVNKVESTPQ PEKSLLMKTL SNFWADRSAY LWKPLVYPTC 1851 PSEHIFTDSD VIIREDEPSS LIAFCLSTSD YRNKMMNLNV QQQQQQQTAE 1901 AAPAKTGGNS GGTTQTGDPS VNISPSVSTT SHNKGRDSEI SSLVTTKEGL 1951 LNTPPIEGAR DRTPQESQTH SQANLDTLQE LEKIMTKKTA THLRYQFEEG 2001 LTVMSCKIFF TEHFDVFRKI CDCQENFIQS LSRCVKWDSN GGKSGSGFLK 2051 TLDDRFIIKE LSHAELEAFI KFAPSYFEYM AQAMFHDLPT TLAKVFGFYQ 2101 IQVKSSISSS KSYKMDVIIM ENLFYEKKTT RIFDLKGSMR NRHVEQTGKA 2151 NEVLLDENMV EYIYESPIHV REYDKKLLRA SVWNDTLFLA KMNVMDYSLV 2201 IGIDNEGYTL TVGIIDFIRT FTWDKKLESW VKEKGLVGGA SVIKQPTVVT 2251 PRQYKKRFRE AMERYILMVP DPWYREGN*

Amino Acid Sequence (or in FASTA format)

       1  MSSEEPHASI SFPDGSHVRS SSTGTSSVNT IDATLSRPNY IKKPSLHIMS
      51  TSTTSTTTDL VTNPILSNIS VPKISPPTSS SIATATSTSH VTGTASHSNI
     101  KANANTSTSV NKKNLPPTTS GRIPSSTIKR YPSRYKPSHS LQLPIKNDSN
     151  FKRSSIYASK STVTAIPIRN NRPISMQNSY ARTPDSDHDD VGDEVSSIKS
     201  ASSSLTASLS KSFLFAFYNN RKKDKTSNNG VLSKEYWMKD ESSKECFSCG
     251  KTFNTFRRKH HCRICGQIFC SSCTLLIDGD RFGCHAKMRV CYNCYEHADT
     301  YEDSSDEEND STMQLNEPRS RSRSRSSNTN PYSHSHSHLH LISQDNHNGT
     351  DLHDPVAATD NPQQQNEVYL LNDDDVQSIM TSGEDSKLFI STPPPPPKMA
     401  IPATKQGGSL EISFDSENDR ALHYQDDNPG RHHHLDSVPT RYTIRDMDNI
     451  SHYDTNSNST LRPHYNTNNS TITINNLNNT TSNNSNYNNT NSNSNINNPA
     501  HSLRRSIFHY VSSNSVNKDS NNSSATPASS AQSSSILDPA NRIIGNYAHR
     551  NYKFKFNYNS KGPSQQNDTA NGNNDNNNNN NNNNNNNNNN SASGIADNNN
     601  IPSNDNGTTF TLDKKKRNPL TKSKSTSAYL EYPLNEEDSS EDEGSMSIYS
     651  VLNDDHKTDN PIRSMRNSTK SFQRAQASLQ RMRFRRKSKS KHFPNNSKSS
     701  IYRDLNFLTN STPNLLSVVS DDNLYDDSSP LQDKASSSAA SRLTDRKFSN
     751  SSGSNNNSNS NSNINTDPWK RIASISGFKL KKEKKRELNE VSLLHMHALL
     801  KQLLNDQEIS NLQEWITLLD GALRKVLRTI LNARDLNTLD FRQTYVKIKR
     851  ISGGSPQNSE YIDGVVFSKA LPSKTMPRHL KNPRILLIMF PLEYQKNNNH
     901  FLSIESVFRQ EREYLDKLVS RLKSLHPDII YVGANVSGYA LELLNDSGIV
     951  VQFNMKPQVI ERIAKLTEAD IAISVDKLAT NIKMGECETF EVKSYIYGNI
    1001  SKTYTFLRGC NPELGGTILL RGDSLENLRK IKQVSEFMVY AIFSLKLESS
    1051  FFNDNFIQLS TDVYLKRAES KKLQVFEGYF ADFLIKFNNR ILTVSPTVDF
    1101  PIPFLLEKAR GLEKKLIERI NQYESESDLD RQTQLNMLQG LESTITKKHL
    1151  GNLIKFLHEM EIENLELEFQ KRSRQWEVSY SSSQNLLGTG SHQSITVLYS
    1201  MVSTKTATPC VGPQIVTIDY FWDSDISIGQ FIENVVGTAR YPCQQGCNGL
    1251  YLDHYRSYVH GSGKVDVLIE KFQTRLPKLK DIILTWSYCK KCGTSTPILQ
    1301  ISEKTWNHSF GKYLEVMFWS YKDSVTGIGK CPHDFTKDHV KYFGYNDLVV
    1351  RLEYSDLEVH ELITPPRKIK WKPHIDIKLK VELYYKILEK INNFYGSVLS
    1401  RLERIKLDSM TKDKVLSGQA KIIELKSNAT EEQKLMLQDL DTFYADSPCD
    1451  QHLPLNLVIK SLYDKAVNWN STFAIFAKSY LPSETDISRI TAKQLKKLFY
    1501  DSSRKDSEDK KSLHDEKAKT RKPEKNELPL EGLKDVEKPK IDSKNTTENR
    1551  DRTNEPQNAV TITTFKDDTP IIPTSGTSHL TVTPSASSVS SSLTPQTEER
    1601  PPISRSGTGI SMTHDKSTRP NIRKMSSDSS LCGLASLANE YSKNNKVSKL
    1651  ATFFDQMHFD ALSKEFELER ERERLQLNKD KYQAIRLQTS TPIVEIYKNV
    1701  KDAVDEPLHS RSSGNNLSSA NVKTLEAPVG EHSRANNCNP PNLDQNLETE
    1751  LENSISQWGE NILNPSGKTT ASTHLNSKPV VKETSENPKS IVRESDNSKS
    1801  EPLPPVITTT TVNKVESTPQ PEKSLLMKTL SNFWADRSAY LWKPLVYPTC
    1851  PSEHIFTDSD VIIREDEPSS LIAFCLSTSD YRNKMMNLNV QQQQQQQTAE
    1901  AAPAKTGGNS GGTTQTGDPS VNISPSVSTT SHNKGRDSEI SSLVTTKEGL
    1951  LNTPPIEGAR DRTPQESQTH SQANLDTLQE LEKIMTKKTA THLRYQFEEG
    2001  LTVMSCKIFF TEHFDVFRKI CDCQENFIQS LSRCVKWDSN GGKSGSGFLK
    2051  TLDDRFIIKE LSHAELEAFI KFAPSYFEYM AQAMFHDLPT TLAKVFGFYQ
    2101  IQVKSSISSS KSYKMDVIIM ENLFYEKKTT RIFDLKGSMR NRHVEQTGKA
    2151  NEVLLDENMV EYIYESPIHV REYDKKLLRA SVWNDTLFLA KMNVMDYSLV
    2201  IGIDNEGYTL TVGIIDFIRT FTWDKKLESW VKEKGLVGGA SVIKQPTVVT
    2251  PRQYKKRFRE AMERYILMVP DPWYREGN*

external links for Fab1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Fab1p

1)	Yamamoto A, et al. (1995) Novel PI(4)P 5-kinase homologue, Fab1p, essential for normal vacuole function and morphology in yeast. Mol Biol Cell 6(5):525-39
2)	Zheng B, et al. (1998) Isolation of yeast mutants defective for localization of vacuolar vital dyes. Proc Natl Acad Sci U S A 95(20):11721-6
3)	Cooke FT, et al. (1998) The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential for vacuole function in S. cerevisiae. Curr Biol 8(22):1219-22
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41