CMK1/YFR014C Protein Information

Standard Name	Cmk1p 1, 2
Systematic Name	Yfr014cp
ORF Classification	Verified
Description	Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	CalModulin dependent protein Kinase 1, 2

Experimental Data
Molecules/cell	1520 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	446
Molecular Weight (Da)	50,296
Isoelectric Point (pI)	6.01

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cmk1p (InterPro)
Physical Interactions	There are 199 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012DC8C \| P27466 MIPS: YFR014C NCBI: 1170625 \| 14318536 \| 218417 \| 3554 \| 836769 \| NP_116669.1 \| NM_001179979.2 GenBank/EMBL/DDBJ: DAA12455.1 \| D50617 \| D90375 \| X57782
External Classifications	EC: 2.7.11.17 [Calcium/calmodulin-dependent protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV 51 VRQAKNTETG EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA 101 FKDWFESKDK FYIITQLAKG GELFDRILKK GKFTEEDAVR ILVEILSAVK 151 YMHSQNIVHR DLKPENLLYI DKSDESPLVV ADFGIAKRLK SDEELLYKPA 201 GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR AERVQDFLDE 251 CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII 301 CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD 351 SDFDEGSQAN GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN 401 TVLAEKEKFL NINRVCSSDS DLPGSDIKSL DEAKEKPEGK DTKTEE*

Amino Acid Sequence (or in FASTA format)

       1  MDDKVSEKES SPKQTEEDSE GKMAHVQPAS YVNKKKYVFG KTLGAGTFGV
      51  VRQAKNTETG EDVAVKILIK KALKGNKVQL EALYDELDIL QRLHHPNIVA
     101  FKDWFESKDK FYIITQLAKG GELFDRILKK GKFTEEDAVR ILVEILSAVK
     151  YMHSQNIVHR DLKPENLLYI DKSDESPLVV ADFGIAKRLK SDEELLYKPA
     201  GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSAFR AERVQDFLDE
     251  CTTGEYPVKF HRPYWDSVSN KAKQFILKAL NLDPSKRPTA AELLEDPWII
     301  CTELKTHNLL PGLKEGLDAR QKFRNSVERV RLNMKIQKLR DLYLEQTESD
     351  SDFDEGSQAN GSVPPLKATD TSQLSKKLSE EEQSKLKSEL TSKAFAQLVN
     401  TVLAEKEKFL NINRVCSSDS DLPGSDIKSL DEAKEKPEGK DTKTEE*

external links for Cmk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Cmk1p

1)	Pausch MH, et al. (1991) Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote. EMBO J 10(6):1511-22
2)	Ohya Y, et al. (1991) Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2. J Biol Chem 266(19):12784-94
3)	Cyert MS (2001) Genetic analysis of calmodulin and its targets in Saccharomyces cerevisiae. Annu Rev Genet 35:647-72
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41