UBP6/YFR010W Protein Information Help

Standard Name Ubp6p 1
Systematic Name Yfr010wp
ORF Classification Verified
Description Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance (1, 2, 3, 4, 5, 6)
Name Description UBiquitin-specific Protease 1
Experimental Data
Molecules/cell 6770 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 499
Molecular Weight (Da) 57,110
Isoelectric Point (pI) 7.14

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ubp6p (InterPro)
Physical Interactions There are 138 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000004F90B | P43593
MIPS: YFR010W
NCBI: 1174863 | 14318532 | 55670444 | 836765 | NP_116665.1 | NM_001179975.1
GenBank/EMBL/DDBJ: DAA12450.1 | D50617
External Classifications EC: 3.1.2.15 [Ubiquitin thiolesterase]
EC: 3.4.19.12 [Ubiquitinyl hydrolase 1]
Amino Acid Sequence (or in FASTA format)
 
       1  MSGETFEFNI RHSGKVYPIT LSTDATSADL KSKAEELTQV PSARQKYMVK
      51  GGLSGEESIK IYPLIKPGST VMLLGTPDAN LISKPAKKNN FIEDLAPEQQ
     101  VQQFAQLPVG FKNMGNTCYL NATLQALYRV NDLRDMILNY NPSQGVSNSG
     151  AQDEEIHKQI VIEMKRCFEN LQNKSFKSVL PIVLLNTLRK CYPQFAERDS
     201  QGGFYKQQDA EELFTQLFHS MSIVFGDKFS EDFRIQFKTT IKDTANDNDI
     251  TVKENESDSK LQCHISGTTN FMRNGLLEGL NEKIEKRSDL TGANSIYSVE
     301  KKISRLPKFL TVQYVRFFWK RSTNKKSKIL RKVVFPFQLD VADMLTPEYA
     351  AEKVKVRDEL RKVEKEKNEK EREIKRRKFD PSSSENVMTP REQYETQVAL
     401  NESEKDQWLE EYKKHFPPNL EKGENPSCVY NLIGVITHQG ANSESGHYQA
     451  FIRDELDENK WYKFNDDKVS VVEKEKIESL AGGGESDSAL ILMYKGFGL*

external links for Ubp6p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ubp6p
1) Park KC, et al.  (1997) Purification and characterization of UBP6, a new ubiquitin-specific protease in Saccharomyces cerevisiae. Arch Biochem Biophys 347(1):78-84
2) Hanna J, et al.  (2003) Ubiquitin depletion as a key mediator of toxicity by translational inhibitors. Mol Cell Biol 23(24):9251-61
3) Chernova TA, et al.  (2003) Pleiotropic effects of Ubp6 loss on drug sensitivities and yeast prion are due to depletion of the free ubiquitin pool. J Biol Chem 278(52):52102-15
4) Guterman A and Glickman MH  (2004) Complementary roles for Rpn11 and Ubp6 in deubiquitination and proteolysis by the proteasome. J Biol Chem 279(3):1729-38
5) Crosas B, et al.  (2006) Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell 127(7):1401-13
6) Torres EM, et al.  (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41