MSH4/YFL003C Protein Information

Standard Name	Msh4p
Systematic Name	Yfl003cp
ORF Classification	Verified
Description	Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein (1, 2)
Name Description	MutS Homolog

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	878
Molecular Weight (Da)	99,224
Isoelectric Point (pI)	7.24

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Msh4p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000530F8 \| P40965 MIPS: YFL003C NCBI: 14318519 \| 308153466 \| 563198 \| 730066 \| 836751 \| NP_116652.1 GenBank/EMBL/DDBJ: DAA12436.1 \| D50617 \| U13999

Amino Acid Sequence (or in FASTA format)
1 MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL 51 KKKTLQIAET TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP 101 SIDCVLCCIY EVPRDISTRI GLCIINCNTG QMYLSDFMDS QIYIRVVHKL 151 QIYQPTEILI PSSSLAPTVS KLATMIKFNV AETVKIEEGS RKCFNSQDGL 201 AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI ISKSSRNLNA 251 FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM 301 GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD 351 KLFSRLLCIN HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI 401 SETKKIFNND AIMEIEKLIN SCINEDCVWA SSAIQLLNQR SYAVKSDSNG 451 LLDVSRQIYK EVKEEFFREV EDLTAKNKIN LDHNYDSARG FYLRIKRQEF 501 TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME EILLLSEETV 551 DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI 601 RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI 651 CIMAQMGSGI PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL 701 DDINTETLLI LDELGRGSSI ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI 751 PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ LTQKSVAIEN SGIRVVKKIF 801 NPDIIAEAYN IHSLLKIAKA RTENEDSNGV VDQKTINQMK RIHNLVAILK 851 ECAGNEKEPL TLGKLKEINS DFIENFEE*

Amino Acid Sequence (or in FASTA format)

       1  MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL
      51  KKKTLQIAET TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP
     101  SIDCVLCCIY EVPRDISTRI GLCIINCNTG QMYLSDFMDS QIYIRVVHKL
     151  QIYQPTEILI PSSSLAPTVS KLATMIKFNV AETVKIEEGS RKCFNSQDGL
     201  AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI ISKSSRNLNA
     251  FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM
     301  GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD
     351  KLFSRLLCIN HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI
     401  SETKKIFNND AIMEIEKLIN SCINEDCVWA SSAIQLLNQR SYAVKSDSNG
     451  LLDVSRQIYK EVKEEFFREV EDLTAKNKIN LDHNYDSARG FYLRIKRQEF
     501  TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME EILLLSEETV
     551  DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI
     601  RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI
     651  CIMAQMGSGI PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL
     701  DDINTETLLI LDELGRGSSI ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI
     751  PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ LTQKSVAIEN SGIRVVKKIF
     801  NPDIIAEAYN IHSLLKIAKA RTENEDSNGV VDQKTINQMK RIHNLVAILK
     851  ECAGNEKEPL TLGKLKEINS DFIENFEE*

external links for Msh4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Msh4p

1)	Ross-Macdonald P and Roeder GS (1994) Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction. Cell 79(6):1069-80
2)	Novak JE, et al. (2001) The budding yeast Msh4 protein functions in chromosome synapsis and the regulation of crossover distribution. Genetics 158(3):1013-25