BLM10/YFL007W Protein Information

Standard Name	Blm10p 1
Systematic Name	Yfl007wp
Alias	Yfl006wp
ORF Classification	Verified
Description	Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 (1, 2, 3, 4, 5, 6)
Name Description	BLeoMycin resistance 2, 7

Experimental Data
Molecules/cell	2600 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	2,143
Molecular Weight (Da)	245,993
Isoelectric Point (pI)	5.77

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Blm10p (InterPro)
Physical Interactions	There are 50 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001D477C \| P43583 MIPS: YFL007W NCBI: 298508250 \| 298508251 \| 298508252 \| 298508253 \| 298508254 \| 298508255 \| 298508466 \| 298508467 \| 298508468 \| 298508469 \| 298508470 \| 298508471 \| 37362646 \| 51013959 \| 83300652 \| 836747 \| 836748 \| NP_116648.2 GenBank/EMBL/DDBJ: DAA12433.1 \| AY693254 \| D50617

Amino Acid Sequence (or in FASTA format)
1 MTANNDDDIK SPIPITNKTL SQLKRFERSP GRPSSSQGEI KRKKSRLYAA 51 DGRPHSPLRA RSATPTLQDQ KLFNGMDSTS LLNERLQHYT LDYVSDRAQH 101 MKNIYDPSSR WFSRSVRPEF PIEEFLPYKT ESHEDQAKYL CHVLVNLYIA 151 ISSLDIQGLI SISSKDLADL KKEVDDLALK TDLFRLSNNT AENDLLGNDI 201 ADYDDAEGLE DELDEYFDLA GPDFNATGKI TAKSATIVNV NHWTNELKNC 251 LHFDFPVALR KSLATVYYYL SLVQGQKVYR QMHVDMFERL VSLDDDRTNF 301 TELLQKQGLL LDHQIMLNFL CEFLPYPDPD YARYELSSKE DLQLFRLLLK 351 HAHNAKPFFD KSKESLLVDT MNFLLSSLAP STMMAVMPIV TSVVPYHYHI 401 HSKIIDYFPF CYSIWSSVSA NVAIDTHMYD FVGSISKDVH NKILSSEHEK 451 DVVGVEFGEF GIFTDDQMTF MFNRLQGHLR TDGQIHSYSR TVKPFVYAIN 501 GSKKDRFFEK LVSLAKAIET FIHPSNNGFW TKPNAKFVHA FIKSYHGRVK 551 YEEDICARGV TNGICLTSFC HEEIVEIFLN IISLGSQNKN PDIANYYISC 601 FAYLLELDPS NAYLIYDKIL IDLYDTLADQ FINSRHRIIS SLKQFTRVIR 651 FIVMDKLYRV HITNVLSMLV SKLDMNDTNL TSNLINGIVS IAAFIPIQDL 701 TGEDDYISFE SDTLPLVQQH FYHIKCGESS KTFRVDDELL NNAFKASTTV 751 FQSMLKVYVE KIFQLVDVDL EDSLVTKINQ TTMILQESMD DKIFNYFASL 801 LQRNFWSNDS FKEKDPNYEL VTIPLAALVR RNNGLSKELV RTLLFHIKEQ 851 IKRGAGSVRS TSEIQQRDVK LVLYLTALND VLRQCHESLL EYSDELITFM 901 KYLYDNVTNP PLDVITSIVI HSALATLCTT EITDCRLFPE DSKIPEKDRW 951 GGLQFDPRRF DKQHLSFQWH VPSSDEITLS ISILESLSEY CINNVEELMK 1001 APRHDSEYGD MIQKYVLVMT HTLSGSSLLF DPDFNKYRTQ SNLSYREKLI 1051 LLKNIRENNC DPQELDIDIE QIRSGKDDED YIESKDIEAG LNAGVSDVVQ 1101 LRDEFPDELI VDEEVVSEMP SGVNTPIAGT HGTDNSAMSS DLAFRDLDIY 1151 TCNYYFGNTT EEKLQNPQYL QVHRVRARIG HFFHKLYVFL STNFENNTNM 1201 FQILLHGLKV WFTDLGQETV FNEDPNAFID VDFLENVQSL SHVNEPFTRT 1251 NFAIRANSLH QSRVLLHSTN RKASKLENLL LVDIIQLATS LYPDIYKPAQ 1301 GTLVHCMKQL VGSYGVVINK IIPSLEKAIK DHDYMKIQVI LNVLLIKKIH 1351 RKLMTDYKDI GRLIFLLIEC CRVNELEIGM YADKILTDIV IGIKIPSSVC 1401 VISDQAFLPL APPDGTINLQ VEAVKLAKKK KREYYLSLLV DLQDKLLDKL 1451 DNEKDMGWKI RMFILRFVTQ IQSNLESKPD KRAVFSIISQ ISTKHPEIIH 1501 LVVKSLLSTC NKIISLSDYE YDITRAYKNE FNPSFVEILD TSTTSFPKTF 1551 TEEMNNFDNP KYFIDLRAYV GWLCWGRLMY VMSPKALKLN LRENELEVLK 1601 TAGHLLTREF LRDVTMNLVQ DNETRGVFSS GNVSFFSLVI LLISSGFCEL 1651 NMSDLFELCE SYYNKDDKAS MIMSVEIVAG LVCGSKFMSV SDLDKRDTFI 1701 ENFLAKCLDY ELNHDAFEIW STLAWWLPAV VDLRRSKTFF CHFINADGMF 1751 DRESDAATHQ TSKIYMLRSI LMSMEFRAPD VGKLFDELVF DHPYDQVRQA 1801 VAKLLTTLVQ NQSNPSISDP TTLLEAERND PDGLGLPLKS VPEKVDAYIK 1851 KQFEIIKNLE DSVVGLNPQQ FIKTDYFYRT STIFYWIKEM ARGPNKVLLV 1901 PYLVDYVLPF LIGLVKHKDV CALASLDPVR LYAGLGYMPI RKNHVAAIVD 1951 YVCSSNVALS SNQTKLQLAF IQHFLSAELL QLTEEEKNKI LEFVVSNLYN 2001 EQFVEVRVRA ASILSDIVHN WKEEQPLLSL IERFAKGLDV NKYTSKERQK 2051 LSKTDIKIHG NVLGLGAIIS AFPYVFPLPP WIPKQLSNLS SWARTSGMTG 2101 QAAKNTISEF KKVRADTWKF DRASFNTEEL EDLEGVLWRS YYA*

Amino Acid Sequence (or in FASTA format)

       1  MTANNDDDIK SPIPITNKTL SQLKRFERSP GRPSSSQGEI KRKKSRLYAA
      51  DGRPHSPLRA RSATPTLQDQ KLFNGMDSTS LLNERLQHYT LDYVSDRAQH
     101  MKNIYDPSSR WFSRSVRPEF PIEEFLPYKT ESHEDQAKYL CHVLVNLYIA
     151  ISSLDIQGLI SISSKDLADL KKEVDDLALK TDLFRLSNNT AENDLLGNDI
     201  ADYDDAEGLE DELDEYFDLA GPDFNATGKI TAKSATIVNV NHWTNELKNC
     251  LHFDFPVALR KSLATVYYYL SLVQGQKVYR QMHVDMFERL VSLDDDRTNF
     301  TELLQKQGLL LDHQIMLNFL CEFLPYPDPD YARYELSSKE DLQLFRLLLK
     351  HAHNAKPFFD KSKESLLVDT MNFLLSSLAP STMMAVMPIV TSVVPYHYHI
     401  HSKIIDYFPF CYSIWSSVSA NVAIDTHMYD FVGSISKDVH NKILSSEHEK
     451  DVVGVEFGEF GIFTDDQMTF MFNRLQGHLR TDGQIHSYSR TVKPFVYAIN
     501  GSKKDRFFEK LVSLAKAIET FIHPSNNGFW TKPNAKFVHA FIKSYHGRVK
     551  YEEDICARGV TNGICLTSFC HEEIVEIFLN IISLGSQNKN PDIANYYISC
     601  FAYLLELDPS NAYLIYDKIL IDLYDTLADQ FINSRHRIIS SLKQFTRVIR
     651  FIVMDKLYRV HITNVLSMLV SKLDMNDTNL TSNLINGIVS IAAFIPIQDL
     701  TGEDDYISFE SDTLPLVQQH FYHIKCGESS KTFRVDDELL NNAFKASTTV
     751  FQSMLKVYVE KIFQLVDVDL EDSLVTKINQ TTMILQESMD DKIFNYFASL
     801  LQRNFWSNDS FKEKDPNYEL VTIPLAALVR RNNGLSKELV RTLLFHIKEQ
     851  IKRGAGSVRS TSEIQQRDVK LVLYLTALND VLRQCHESLL EYSDELITFM
     901  KYLYDNVTNP PLDVITSIVI HSALATLCTT EITDCRLFPE DSKIPEKDRW
     951  GGLQFDPRRF DKQHLSFQWH VPSSDEITLS ISILESLSEY CINNVEELMK
    1001  APRHDSEYGD MIQKYVLVMT HTLSGSSLLF DPDFNKYRTQ SNLSYREKLI
    1051  LLKNIRENNC DPQELDIDIE QIRSGKDDED YIESKDIEAG LNAGVSDVVQ
    1101  LRDEFPDELI VDEEVVSEMP SGVNTPIAGT HGTDNSAMSS DLAFRDLDIY
    1151  TCNYYFGNTT EEKLQNPQYL QVHRVRARIG HFFHKLYVFL STNFENNTNM
    1201  FQILLHGLKV WFTDLGQETV FNEDPNAFID VDFLENVQSL SHVNEPFTRT
    1251  NFAIRANSLH QSRVLLHSTN RKASKLENLL LVDIIQLATS LYPDIYKPAQ
    1301  GTLVHCMKQL VGSYGVVINK IIPSLEKAIK DHDYMKIQVI LNVLLIKKIH
    1351  RKLMTDYKDI GRLIFLLIEC CRVNELEIGM YADKILTDIV IGIKIPSSVC
    1401  VISDQAFLPL APPDGTINLQ VEAVKLAKKK KREYYLSLLV DLQDKLLDKL
    1451  DNEKDMGWKI RMFILRFVTQ IQSNLESKPD KRAVFSIISQ ISTKHPEIIH
    1501  LVVKSLLSTC NKIISLSDYE YDITRAYKNE FNPSFVEILD TSTTSFPKTF
    1551  TEEMNNFDNP KYFIDLRAYV GWLCWGRLMY VMSPKALKLN LRENELEVLK
    1601  TAGHLLTREF LRDVTMNLVQ DNETRGVFSS GNVSFFSLVI LLISSGFCEL
    1651  NMSDLFELCE SYYNKDDKAS MIMSVEIVAG LVCGSKFMSV SDLDKRDTFI
    1701  ENFLAKCLDY ELNHDAFEIW STLAWWLPAV VDLRRSKTFF CHFINADGMF
    1751  DRESDAATHQ TSKIYMLRSI LMSMEFRAPD VGKLFDELVF DHPYDQVRQA
    1801  VAKLLTTLVQ NQSNPSISDP TTLLEAERND PDGLGLPLKS VPEKVDAYIK
    1851  KQFEIIKNLE DSVVGLNPQQ FIKTDYFYRT STIFYWIKEM ARGPNKVLLV
    1901  PYLVDYVLPF LIGLVKHKDV CALASLDPVR LYAGLGYMPI RKNHVAAIVD
    1951  YVCSSNVALS SNQTKLQLAF IQHFLSAELL QLTEEEKNKI LEFVVSNLYN
    2001  EQFVEVRVRA ASILSDIVHN WKEEQPLLSL IERFAKGLDV NKYTSKERQK
    2051  LSKTDIKIHG NVLGLGAIIS AFPYVFPLPP WIPKQLSNLS SWARTSGMTG
    2101  QAAKNTISEF KKVRADTWKF DRASFNTEEL EDLEGVLWRS YYA*

external links for Blm10p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Blm10p

1)	Doherty K, et al. (2004) Expression of the expanded YFL007w ORF and assignment of the gene name BLM10. Yeast 21(12):1021-3
2)	Febres DE, et al. (2001) The novel BLM3 gene encodes a protein that protects against lethal effects of oxidative damage. Cell Mol Biol (Noisy-le-grand) 47(7):1149-62
3)	Fehlker M, et al. (2003) Blm3 is part of nascent proteasomes and is involved in a late stage of nuclear proteasome assembly. EMBO Rep 4(10):959-63
4)	Schmidt M, et al. (2005) The HEAT repeat protein Blm10 regulates the yeast proteasome by capping the core particle. Nat Struct Mol Biol 12(4):294-303
5)	Ustrell V, et al. (2002) PA200, a nuclear proteasome activator involved in DNA repair. EMBO J 21(13):3516-25
6)	Dange T, et al. (2011) Blm10 protein promotes proteasomal substrate turnover by an active gating mechanism. J Biol Chem 286(50):42830-9
7)	Moore CW (1991) Further characterizations of bleomycin-sensitive (blm) mutants of Saccharomyces cerevisiae with implications for a radiomimetic model. J Bacteriol 173(11):3605-8
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41