CDC4/YFL009W Protein Information Help

Standard Name Cdc4p
Systematic Name Yfl009wp
ORF Classification Verified
Description F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p; Cdc4p interacts with G1 cyclin Cln3p, and Cdc4p and Grr1p redundantly target Cln3p for degradation. (1, 2, 3, 4)
Name Description Cell Division Cycle 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 779
Molecular Weight (Da) 86,089
Isoelectric Point (pI) 7.14

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cdc4p (InterPro)
Physical Interactions There are 99 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000127254 | P07834
MIPS: YFL009W
NCBI: 1168815 | 14318513 | 150261362 | 150261363 | 150261364 | 150261365 | 28948827 | 28948829 | 301015909 | 301015911 | 3503 | 385867621 | 385867623 | 559926 | 836745 | NP_116585.1
GenBank/EMBL/DDBJ: DAA12431.1 | D50617 | X05625 | Z46255
Amino Acid Sequence (or in FASTA format)
 
       1  MGSFPLAEFP LRDIPVPYSY RVSGGIASSG SVTALVTAAG THRNSSTAKT
      51  VETEDGEEDI DEYQRKRAAG SGESTPERSD FKRVKHDNHK TLHPVNLQNT
     101  GAASVDNDGL HNLTDISNDA EKLLMSVDDG SAAPSTLSVN MGVASHNVAA
     151  PTTVNAATIT GSDVSNNVNS ATINNPMEEG ALPLSPTASS PGTTTPLAKT
     201  TKTINNNNNI ADLIESKDSI ISPEYLSDEI FSAINNNLPH AYFKNLLFRL
     251  VANMDRSELS DLGTLIKDNL KRDLITSLPF EISLKIFNYL QFEDIINSLG
     301  VSQNWNKIIR KSTSLWKKLL ISENFVSPKG FNSLNLKLSQ KYPKLSQQDR
     351  LRLSFLENIF ILKNWYNPKF VPQRTTLRGH MTSVITCLQF EDNYVITGAD
     401  DKMIRVYDSI NKKFLLQLSG HDGGVWALKY AHGGILVSGS TDRTVRVWDI
     451  KKGCCTHVFK GHNSTVRCLD IVEYKNIKYI VTGSRDNTLH VWKLPKESSV
     501  PDHGEEHDYP LVFHTPEENP YFVGVLRGHM ASVRTVSGHG NIVVSGSYDN
     551  TLIVWDVAQM KCLYILSGHT DRIYSTIYDH ERKRCISASM DTTIRIWDLE
     601  NIWNNGECSY ATNSASPCAK ILGAMYTLQG HTALVGLLRL SDKFLVSAAA
     651  DGSIRGWDAN DYSRKFSYHH TNLSAITTFY VSDNILVSGS ENQFNIYNLR
     701  SGKLVHANIL KDADQIWSVN FKGKTLVAAV EKDGQSFLEI LDFSKASKIN
     751  YVSNPVNSSS SSLESISTSL GLTRTTIIP*

external links for Cdc4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cdc4p
1) Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30
2) Goh PY and Surana U  (1999) Cdc4, a protein required for the onset of S phase, serves an essential function during G(2)/M transition in Saccharomyces cerevisiae. Mol Cell Biol 19(8):5512-22
3) Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19
4) Landry BD, et al.  (2012) F-box protein specificity for g1 cyclins is dictated by subcellular localization. PLoS Genet 8(7):e1002851
5) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9