HSP12/YFL014W Protein Information Help

Standard Name Hsp12p
Systematic Name Yfl014wp
Alias Glp1p , Hor5p 1
ORF Classification Verified
Description Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension (1, 10, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Heat Shock Protein 5
Experimental Data
Molecules/cell 4490 11
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 109
Molecular Weight (Da) 11,693
Isoelectric Point (pI) 5.1

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hsp12p (InterPro)
Physical Interactions There are 12 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000036C44 | P22943
MIPS: YFL014W
NCBI: 123537 | 14318506 | 171607 | 1742028 | 18479209 | 340707296 | 350610334 | 3800 | 400260720 | 45270818 | 559934 | 836740 | NP_116640.1
GenBank/EMBL/DDBJ: DAA12425.1 | AY046957 | AY558464 | D50617 | D89864 | M60827 | X55785 | Z46255
Amino Acid Sequence (or in FASTA format)
 
       1  MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK
      51  GVFQGVHDSA EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV
     101  HGEEDPTKK*

external links for Hsp12p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome

YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Hsp12p
1) Hirayama T, et al.  (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
2) Stone RL, et al.  (1990) Cloning, sequencing and chromosomal assignment of a gene from Saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids. Gene 96(2):171-6
3) Siderius M, et al.  (1997) High-osmolarity signalling in Saccharomyces cerevisiae is modulated in a carbon-source-dependent fashion. Microbiology 143 ( Pt 10):3241-50
4) Rep M, et al.  (1999) Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol Cell Biol 19(8):5474-85
5) Praekelt UM and Meacock PA  (1990) HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function. Mol Gen Genet 223(1):97-106
6) Garay-Arroyo A, et al.  (2000) Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. J Biol Chem 275(8):5668-74
7) Sales K, et al.  (2000) The LEA-like protein HSP 12 in Saccharomyces cerevisiae has a plasma membrane location and protects membranes against desiccation and ethanol-induced stress. Biochim Biophys Acta 1463(2):267-78
8) Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520
9) Herbert AP, et al.  (2012) NMR structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast. PLoS One 7(7):e41975
10) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
11) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41