STE2/YFL026W Protein Information

Standard Name	Ste2p
Systematic Name	Yfl026wp
ORF Classification	Verified
Description	Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells (1, 2, 3)
Name Description	STErile

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	431
Molecular Weight (Da)	47,849
Isoelectric Point (pI)	6.95

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ste2p (InterPro)
Transmembrane Domains	There are 7 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 28 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001360D9 \| D6VTK4 MIPS: YFL026W NCBI: 172749 \| 229597563 \| 330443535 \| 347595716 \| 37927486 \| 4558 \| 45720076 \| 51013719 \| 836728 \| NP_116627.2 GenBank/EMBL/DDBJ: DAA12414.2 \| AJ585736 \| AY693134 \| D50617 \| M24335 \| X03010

Amino Acid Sequence (or in FASTA format)
1 MSDAAPSLSN LFYDPTYNPG QSTINYTSIY GNGSTITFDE LQGLVNSTVT 51 QAIMFGVRCG AAALTLIVMW MTSRSRKTPI FIINQVSLFL IILHSALYFK 101 YLLSNYSSVT YALTGFPQFI SRGDVHVYGA TNIIQVLLVA SIETSLVFQI 151 KVIFTGDNFK RIGLMLTSIS FTLGIATVTM YFVSAVKGMI VTYNDVSATQ 201 DKYFNASTIL LASSINFMSF VLVVKLILAI RSRRFLGLKQ FDSFHILLIM 251 SCQSLLVPSI IFILAYSLKP NQGTDVLTTV ATLLAVLSLP LSSMWATAAN 301 NASKTNTITS DFTTSTDRFY PGTLSSFQTD SINNDAKSSL RSRLYDLYPR 351 RKETTSDKHS ERTFVSETAD DIEKNQFYQL PTPTSSKNTR IGPFADASYK 401 EGEVEPVDMY TPDTAADEEA RKFWTEDNNN L*

Amino Acid Sequence (or in FASTA format)

       1  MSDAAPSLSN LFYDPTYNPG QSTINYTSIY GNGSTITFDE LQGLVNSTVT
      51  QAIMFGVRCG AAALTLIVMW MTSRSRKTPI FIINQVSLFL IILHSALYFK
     101  YLLSNYSSVT YALTGFPQFI SRGDVHVYGA TNIIQVLLVA SIETSLVFQI
     151  KVIFTGDNFK RIGLMLTSIS FTLGIATVTM YFVSAVKGMI VTYNDVSATQ
     201  DKYFNASTIL LASSINFMSF VLVVKLILAI RSRRFLGLKQ FDSFHILLIM
     251  SCQSLLVPSI IFILAYSLKP NQGTDVLTTV ATLLAVLSLP LSSMWATAAN
     301  NASKTNTITS DFTTSTDRFY PGTLSSFQTD SINNDAKSSL RSRLYDLYPR
     351  RKETTSDKHS ERTFVSETAD DIEKNQFYQL PTPTSSKNTR IGPFADASYK
     401  EGEVEPVDMY TPDTAADEEA RKFWTEDNNN L*

external links for Ste2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Ste2p

1)	Blumer KJ and Thorner J (1990) Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the alpha subunit but are required for receptor coupling. Proc Natl Acad Sci U S A 87(11):4363-7
2)	Burkholder AC and Hartwell LH (1985) The yeast alpha-factor receptor: structural properties deduced from the sequence of the STE2 gene. Nucleic Acids Res 13(23):8463-75
3)	Jenness DD, et al. (1986) Binding of alpha-factor pheromone to Saccharomyces cerevisiae a cells: dissociation constant and number of binding sites. Mol Cell Biol 6(1):318-20