CAK1/YFL029C Protein Information

Standard Name	Cak1p
Systematic Name	Yfl029cp
Alias	Civ1p 1
ORF Classification	Verified
Description	Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases (2, 3)
Name Description	Cdk-Activating Kinase 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	368
Molecular Weight (Da)	42,186
Isoelectric Point (pI)	5.71

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cak1p (InterPro)
Physical Interactions	There are 21 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000126E24 \| P43568 MIPS: YFL029C NCBI: 1170645 \| 14318490 \| 1480664 \| 836725 \| NP_116624.1 GenBank/EMBL/DDBJ: DAA12411.1 \| D50617 \| U60192
External Classifications	EC: 2.7.11.22 [Cyclin-dependent kinase]

Amino Acid Sequence (or in FASTA format)
1 MKLDSIDITH CQLVKSTRTA RIYRSDTYAI KCLALDFDIP PHNAKFEVSI 51 LNKLGNKCKH ILPLLESKAT DNNDLLLLFP FEEMNLYEFM QMHYKRDRRK 101 KNPYYDLLNP SIPIVADPPV QKYTNQLDVN RYSLSFFRQM VEGIAFLHEN 151 KIIHRDIKPQ NIMLTNNTST VSPKLYIIDF GISYDMANNS QTSAEPMDSK 201 VTDISTGIYK APEVLFGVKC YDGGVDVWSL LIIISQWFQR ETSRMGHVPA 251 MIDDGSDDMN SDGSDFRLIC SIFEKLGIPS IQKWEEVAQH GSVDAFVGMF 301 GADGDGKYVL DQEKDVQISI VERNMPRLDE IADVKVKQKF INCILGMVSF 351 SPNERWSCQR ILQELEKP*

Amino Acid Sequence (or in FASTA format)

       1  MKLDSIDITH CQLVKSTRTA RIYRSDTYAI KCLALDFDIP PHNAKFEVSI
      51  LNKLGNKCKH ILPLLESKAT DNNDLLLLFP FEEMNLYEFM QMHYKRDRRK
     101  KNPYYDLLNP SIPIVADPPV QKYTNQLDVN RYSLSFFRQM VEGIAFLHEN
     151  KIIHRDIKPQ NIMLTNNTST VSPKLYIIDF GISYDMANNS QTSAEPMDSK
     201  VTDISTGIYK APEVLFGVKC YDGGVDVWSL LIIISQWFQR ETSRMGHVPA
     251  MIDDGSDDMN SDGSDFRLIC SIFEKLGIPS IQKWEEVAQH GSVDAFVGMF
     301  GADGDGKYVL DQEKDVQISI VERNMPRLDE IADVKVKQKF INCILGMVSF
     351  SPNERWSCQR ILQELEKP*

external links for Cak1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cak1p

1)	Thuret JY, et al. (1996) Civ1 (CAK in vivo), a novel Cdk-activating kinase. Cell 86(4):565-76
2)	Enke DA, et al. (1999) The CDK-activating kinase (Cak1p) from budding yeast has an unusual ATP-binding pocket. J Biol Chem 274(4):1949-56
3)	Enke DA, et al. (2000) Kinetic analysis of the cyclin-dependent kinase-activating kinase (Cak1p) from budding yeast. J Biol Chem 275(43):33267-71
4)	Kaldis P, et al. (2000) The effects of changing the site of activating phosphorylation in CDK2 from threonine to serine. J Biol Chem 275(42):32578-84