RIM15/YFL033C Protein Information

Standard Name	Rim15p 1
Systematic Name	Yfl033cp
Alias	Tak1p
ORF Classification	Verified
Description	Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase (2, 3, 4, 5)
Name Description	Regulator of IME2 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,770
Molecular Weight (Da)	196,529
Isoelectric Point (pI)	6.43

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rim15p (InterPro)
Physical Interactions	There are 35 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001338B8 \| P43565 MIPS: YFL033C NCBI: 1170646 \| 14318486 \| 2213667 \| 3250892 \| 836721 \| NP_116620.1 GenBank/EMBL/DDBJ: DAA12408.1 \| AJ001030 \| D50617 \| U83459
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MFNRSNTAGG SQAMKEGLGI NKLSPISSNS NPSSLTSSNY EKYLQLATEK 51 NPCMILELEL DGKVRYGSPQ WNTITGVADD SGSSPTYIAD LILGSDQDKG 101 VFQKATDMLL MNDDTSCTIT FKIKAADYEG SAGCDDESTI TTLEARGILI 151 RDGHTQLPSH TMWIVKPRTN DWSDFYANED AQDDMVIQLS DNCDDIDIQL 201 PEEFAKTLGF GAKIFVQYLK RIRLEMIIDE FNLPLPKMEL CRVCENFVPV 251 WWLETHSQSC VCEHRTESLI QLLHDNLLEQ QAILANFTKD SEYKGSQIQV 301 RSNNFLNQVL DSLRELCQDA IDINPSEMVP DLYHSLSTFP QDNGNNNNNN 351 NNNNNNNNAL LDQFPIQKDT VSLNSYFQFS PRTNHNIQNV TSWQSRFFLN 401 DDQDPGLALL IHDTLDLARK KVDAVLRLDN AMTYSLKIKN EVNNYVVQLI 451 REQIEINKHA ILTHPMNLRS SSIFHSPLPQ IHSQQPEAEN LIYSSSTPLQ 501 VQHDQCASFE APSKSHLEPI PFPVSSIEET PTANDIRHPS PLPRSCSNTV 551 MKLPTPRRKL DSNGLFSDAY LNADIIPNPS IESTISIDRD NNTNSRGSSM 601 KQYGIGEATD SRTSNSERPS SSSSRLGIRS RSITPRQKIE YSHVDNDDRT 651 NEMLSRDKDS LQPQPSVDTT ITSSTQATTT GTKTNSNNST NSVLPKLMTS 701 ISLTPRRGSP SFGNLASHSM QQTNSFKLIH DKSPISSPFT FSKDFLTPEQ 751 HPSNIARTDS INNAMLTSPN MPLSPLLLAT NQTVKSPTPS IKDYDILKPI 801 SKGAYGSVYL ARKKLTGDYF AIKVLRKSDM IAKNQVTNVK SERAIMMVQS 851 DKPYVARLFA SFQNKDNLFL VMEYLPGGDL ATLIKMMGYL PDQWAKQYLT 901 EIVVGVNDMH QNGIIHHDLK PENLLIDNAG HVKLTDFGLS RAGLIRRHKF 951 VPHKSSLSIS STLPIDNPAN NFTMNNNNSN HSQLSTPDSF TSDHKQYNRS 1001 KKSSLGQQYE HSEYSSTSNS HSMTPTPSTN TVVYPSYYRG KDRSHGSSNI 1051 DLPASLRRSE SQLSFSLLDI SRSSTPPLAN PTNSNANNIM RRKSLTENKS 1101 FSNDLLSSDA IAATNTNINS NNNISLSPAP SDLALFYPDD SKQNKKFFGT 1151 PDYLAPETIE GKGEDNKQCD WWSVGCIFFE LLLGYPPFHA ETPDAVFKKI 1201 LSGVIQWPEF KNEEEEREFL TPEAKDLIEK LLVVDPAKRL GAKGIQEIKD 1251 HPYFKNVDWD HVYDEEASFV PTIDNPEDTD YFDLRGAELQ DFGDDIENDN 1301 ANILFGKHGI NTDVSELSAA NLSPPLNHKN ILSRKLSMSN TTNRSSNNSN 1351 SSVHDFGAHT PVNKLSIASV LESVPQETGY ITPNGTGTTT TSAKNSPNLK 1401 NLSLAIPPHM RDRRSSKLND SQTEFGSFNF RNLSALDKAN KDAINRLKSE 1451 HFSEQPGVHR RTSSASLMGS SSDGSVSTPG SNASNTTSGG KLKIHKPTIS 1501 GSPSTFGTFP KTFLRSDSFS TRSYSPERSI SIDSSTLSRK GSIIGDNQQT 1551 TANSSDSPTM TKFKSPLSPA NTTTVSSYFS RQRVLSKSFS QRTNSSDLSA 1601 EESDRLQAIS RVNSLRNRRR SGRKSSSTSE IGYHMDVLVC EPIPIHRYRV 1651 TKDLENLGCT VVSVGAGDEL VSRATSGVSF DLIMTALKLP KLGAIDIVQL 1701 LKQTNGANST TPIVAITNYF QEAATSRVFD DVLEKPVKLD ELKKLVAKYA 1751 LKKSQEDEEH TILSDSDETH *

Amino Acid Sequence (or in FASTA format)

       1  MFNRSNTAGG SQAMKEGLGI NKLSPISSNS NPSSLTSSNY EKYLQLATEK
      51  NPCMILELEL DGKVRYGSPQ WNTITGVADD SGSSPTYIAD LILGSDQDKG
     101  VFQKATDMLL MNDDTSCTIT FKIKAADYEG SAGCDDESTI TTLEARGILI
     151  RDGHTQLPSH TMWIVKPRTN DWSDFYANED AQDDMVIQLS DNCDDIDIQL
     201  PEEFAKTLGF GAKIFVQYLK RIRLEMIIDE FNLPLPKMEL CRVCENFVPV
     251  WWLETHSQSC VCEHRTESLI QLLHDNLLEQ QAILANFTKD SEYKGSQIQV
     301  RSNNFLNQVL DSLRELCQDA IDINPSEMVP DLYHSLSTFP QDNGNNNNNN
     351  NNNNNNNNAL LDQFPIQKDT VSLNSYFQFS PRTNHNIQNV TSWQSRFFLN
     401  DDQDPGLALL IHDTLDLARK KVDAVLRLDN AMTYSLKIKN EVNNYVVQLI
     451  REQIEINKHA ILTHPMNLRS SSIFHSPLPQ IHSQQPEAEN LIYSSSTPLQ
     501  VQHDQCASFE APSKSHLEPI PFPVSSIEET PTANDIRHPS PLPRSCSNTV
     551  MKLPTPRRKL DSNGLFSDAY LNADIIPNPS IESTISIDRD NNTNSRGSSM
     601  KQYGIGEATD SRTSNSERPS SSSSRLGIRS RSITPRQKIE YSHVDNDDRT
     651  NEMLSRDKDS LQPQPSVDTT ITSSTQATTT GTKTNSNNST NSVLPKLMTS
     701  ISLTPRRGSP SFGNLASHSM QQTNSFKLIH DKSPISSPFT FSKDFLTPEQ
     751  HPSNIARTDS INNAMLTSPN MPLSPLLLAT NQTVKSPTPS IKDYDILKPI
     801  SKGAYGSVYL ARKKLTGDYF AIKVLRKSDM IAKNQVTNVK SERAIMMVQS
     851  DKPYVARLFA SFQNKDNLFL VMEYLPGGDL ATLIKMMGYL PDQWAKQYLT
     901  EIVVGVNDMH QNGIIHHDLK PENLLIDNAG HVKLTDFGLS RAGLIRRHKF
     951  VPHKSSLSIS STLPIDNPAN NFTMNNNNSN HSQLSTPDSF TSDHKQYNRS
    1001  KKSSLGQQYE HSEYSSTSNS HSMTPTPSTN TVVYPSYYRG KDRSHGSSNI
    1051  DLPASLRRSE SQLSFSLLDI SRSSTPPLAN PTNSNANNIM RRKSLTENKS
    1101  FSNDLLSSDA IAATNTNINS NNNISLSPAP SDLALFYPDD SKQNKKFFGT
    1151  PDYLAPETIE GKGEDNKQCD WWSVGCIFFE LLLGYPPFHA ETPDAVFKKI
    1201  LSGVIQWPEF KNEEEEREFL TPEAKDLIEK LLVVDPAKRL GAKGIQEIKD
    1251  HPYFKNVDWD HVYDEEASFV PTIDNPEDTD YFDLRGAELQ DFGDDIENDN
    1301  ANILFGKHGI NTDVSELSAA NLSPPLNHKN ILSRKLSMSN TTNRSSNNSN
    1351  SSVHDFGAHT PVNKLSIASV LESVPQETGY ITPNGTGTTT TSAKNSPNLK
    1401  NLSLAIPPHM RDRRSSKLND SQTEFGSFNF RNLSALDKAN KDAINRLKSE
    1451  HFSEQPGVHR RTSSASLMGS SSDGSVSTPG SNASNTTSGG KLKIHKPTIS
    1501  GSPSTFGTFP KTFLRSDSFS TRSYSPERSI SIDSSTLSRK GSIIGDNQQT
    1551  TANSSDSPTM TKFKSPLSPA NTTTVSSYFS RQRVLSKSFS QRTNSSDLSA
    1601  EESDRLQAIS RVNSLRNRRR SGRKSSSTSE IGYHMDVLVC EPIPIHRYRV
    1651  TKDLENLGCT VVSVGAGDEL VSRATSGVSF DLIMTALKLP KLGAIDIVQL
    1701  LKQTNGANST TPIVAITNYF QEAATSRVFD DVLEKPVKLD ELKKLVAKYA
    1751  LKKSQEDEEH TILSDSDETH *

external links for Rim15p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rim15p

1)	Su SS and Mitchell AP (1993) Identification of functionally related genes that stimulate early meiotic gene expression in yeast. Genetics 133(1):67-77
2)	Vidan S and Mitchell AP (1997) Stimulation of yeast meiotic gene expression by the glucose-repressible protein kinase Rim15p. Mol Cell Biol 17(5):2688-97
3)	Cameroni E, et al. (2004) The novel yeast PAS kinase Rim 15 orchestrates G0-associated antioxidant defense mechanisms. Cell Cycle 3(4):462-8
4)	Roosen J, et al. (2005) PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability. Mol Microbiol 55(3):862-80
5)	Wanke V, et al. (2005) Regulation of G0 entry by the Pho80-Pho85 cyclin-CDK complex. EMBO J 24(24):4271-8