RPO41/YFL036W Protein Information Help

Standard Name Rpo41p
Systematic Name Yfl036wp
ORF Classification Verified
Description Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation (1, 2, 3)
Name Description RNA POlymerase
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,351
Molecular Weight (Da) 153,046
Isoelectric Point (pI) 9.55

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rpo41p (InterPro)
Physical Interactions There are 23 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000168318 | P13433
MIPS: YFL036W
NCBI: 133457 | 14318482 | 172490 | 308153492 | 836718 | NP_116617.1
GenBank/EMBL/DDBJ: DAA12404.1 | D50617 | M17539
External Classifications EC: 2.7.7.6 [DNA-directed RNA polymerase]
Amino Acid Sequence (or in FASTA format)
 
       1  MLRPAYKSLV KTSLLQRRLI SSKGSKLFKP SPDSTSTILI SEDPLVTGSS
      51  PTSSTTSGII SSNDFPLFNK NRKDAKSSIS YQWKNPSELE FDPFNKSHAS
     101  AVTSMTRTRD VMQLWSLLEA CLQSNLMKRA FSILESLYLV PEHKQRFIED
     151  YNMYLNSFSK NDPNFPILKM NEKLTNDLET SFKDVNYNDK TLAIMIHHAL
     201  NFHSTTSSML LKPIISAYLK MSVNGIREIF SCLDILTISD LNILMNDLKV
     251  ITPSQLPNSV RPILESLTLS PTPVNNIENE EGLNKVEAEN DSKLHKASNA
     301  SSDSIKKPSL DPLREVSFHG STEVLSKDAE KLIAVDTIGM RVIRHTLLGL
     351  SLTPEQKEQI SKFKFDANDN VLKMKPTKND DNNNSINFFE IYNSLPTLEE
     401  KKAFESALNI FNQDRQKVLE NRATEAARER WKHDFEEAKA RGDISIEKNL
     451  NVKLWKWYNE MLPLVKEEIN HCRSLLSEKL SDKKGLNKVD TNRLGYGPYL
     501  TLIDPGKMCV ITILELLKLN STGGVIEGMR TARAVISVGK AIEMEFRSEQ
     551  VLKSESQAFR DVNKKSPEFK KLVQNAKSVF RSSQIEQSKI LWPQSIRARI
     601  GSVLISMLIQ VAKVSVQGVD PVTKAKVHGE APAFAHGYQY HNGSKLGVLK
     651  IHKTLIRQLN GERLIASVQP QLLPMLVEPK PWVNWRSGGY HYTQSTLLRT
     701  KDSPEQVAYL KAASDNGDID RVYDGLNVLG RTPWTVNRKV FDVVSQVWNK
     751  GEGFLDIPGA QDEMVLPPAP PKNSDPSILR AWKLQVKTIA NKFSSDRSNR
     801  CDTNYKLEIA RAFLGEKLYF PHNLDFRGRA YPLSPHFNHL GNDMSRGLLI
     851  FWHGKKLGPS GLKWLKIHLS NLFGFDKLPL KDRVAFTESH LQDIKDSAEN
     901  PLTGDRWWTT ADKPWQALAT CFELNEVMKM DNPEEFISHQ PVHQDGTCNG
     951  LQHYAALGGD VEGATQVNLV PSDKPQDVYA HVARLVQKRL EIAAEKGDEN
    1001  AKILKDKITR KVVKQTVMTN VYGVTYVGAT FQIAKQLSPI FDDRKESLDF
    1051  SKYLTKHVFS AIRELFHSAH LIQDWLGESA KRISKSIRLD VDEKSFKNGN
    1101  KPDFMSSVIW TTPLGLPIVQ PYREESKKQV ETNLQTVFIS DPFAVNPVNA
    1151  RRQKAGLPPN FIHSLDASHM LLSAAECGKQ GLDFASVHDS YWTHASDIDT
    1201  MNVVLREQFI KLHEVDLVLR LKEEFDQRYK NYVKIGKLKR STDLAQKIIR
    1251  IRKDLSRKLG RSTTLADEIY FEKKRQELLN SPLIEDRNVG EKMVTTVSLF
    1301  EDITDLDALE LENGGDENSG MSVLLPLRLP EIPPKGDFDV TVLRNSQYFF
    1351  S*

external links for Rpo41p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Rpo41p
1) Greenleaf AL, et al.  (1986) Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome. Proc Natl Acad Sci U S A 83(10):3391-4
2) Tang GQ, et al.  (2011) Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase. J Biol Chem 286(44):38805-13
3) Velazquez G, et al.  (2012) Conservation of promoter melting mechanisms in divergent regions of the single-subunit RNA polymerases. Biochemistry 51(18):3901-10