SEC53/YFL045C Protein Information

Standard Name	Sec53p 1
Systematic Name	Yfl045cp
Alias	Alg4p 2
ORF Classification	Verified
Description	Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen (3, 4)
Name Description	SECretory 1

Experimental Data
Molecules/cell	3500 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	254
Molecular Weight (Da)	29,063
Isoelectric Point (pI)	4.99

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sec53p (InterPro)
Physical Interactions	There are 37 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000302B4 \| P07283 MIPS: YFL045C NCBI: 134285 \| 14318474 \| 4445 \| 45720044 \| 45720046 \| 45720048 \| 45720050 \| 45720052 \| 45720054 \| 45720056 \| 45720058 \| 45720060 \| 45720062 \| 45720064 \| 45720066 \| 45720068 \| 45720070 \| 45720072 \| 45720074 \| 51013369 \| 836710 \| NP_116609.1 \| NM_001179922.1 GenBank/EMBL/DDBJ: DAA12395.1 \| AJ585720 \| AJ585721 \| AJ585722 \| AJ585723 \| AJ585724 \| AJ585725 \| AJ585726 \| AJ585727 \| AJ585728 \| AJ585729 \| AJ585730 \| AJ585731 \| AJ585732 \| AJ585733 \| AJ585734 \| AJ585735 \| AY692959 \| D50617 \| X03213
External Classifications	EC: 5.4.2.8 [Phosphomannomutase]

Amino Acid Sequence (or in FASTA format)
1 MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF 51 VGGSDLSKQL EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE 101 EKYNKLAVFI LRYLSEIDLP KRRGTFLEFR NGMINVSPIG RNASTEERNE 151 FERYDKEHQI RAKFVEALKK EFPDYGLTFS IGGQISFDVF PAGWDKTYCL 201 QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS PDDTVKILTE 251 LFNL*

Amino Acid Sequence (or in FASTA format)

       1  MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF
      51  VGGSDLSKQL EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE
     101  EKYNKLAVFI LRYLSEIDLP KRRGTFLEFR NGMINVSPIG RNASTEERNE
     151  FERYDKEHQI RAKFVEALKK EFPDYGLTFS IGGQISFDVF PAGWDKTYCL
     201  QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS PDDTVKILTE
     251  LFNL*

external links for Sec53p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Sec53p

1)	Bernstein M, et al. (1985) Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum. J Cell Biol 101(6):2374-82
2)	Huffaker TC and Robbins PW (1983) Yeast mutants deficient in protein glycosylation. Proc Natl Acad Sci U S A 80(24):7466-70
3)	Kepes F and Schekman R (1988) The yeast SEC53 gene encodes phosphomannomutase. J Biol Chem 263(19):9155-61
4)	Feldman RI, et al. (1987) Product of SEC53 is required for folding and glycosylation of secretory proteins in the lumen of the yeast endoplasmic reticulum. J Biol Chem 262(19):9332-9
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41