RGD2/YFL047W Protein Information

Standard Name	Rgd2p
Systematic Name	Yfl047wp
ORF Classification	Verified
Description	GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress (1, 2)
Name Description	Related GAP Domain 1

Experimental Data
Molecules/cell	1750 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	714
Molecular Weight (Da)	82,208
Isoelectric Point (pI)	5.64

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rgd2p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013AE3C \| P43556 MIPS: YFL047W NCBI: 1175952 \| 16198342 \| 836708 \| NP_428268.1 GenBank/EMBL/DDBJ: DAA12393.1 \| D50617

Amino Acid Sequence (or in FASTA format)
1 MLSFCDYFWS EDLVSGLDVL FDRLYHGCEQ CDLFIQLFAS RMQFEVSHGR 51 QLFGIEAGMD NLKAVQEDED EGVTVSRALR GILQEMSQEG THHLTIASNI 101 ESLVLQPFSK WCIEHRERIQ YSEKTLLTNV NNFRKSKKYV GKLEKEYFNK 151 CRQLEEFKRT HFNEDELANA MKSLKIQNKY EEDVAREKDH RFFNRIAGID 201 FDYKTMKETL QLLLTKLPKT DYKLPLISYS LSNTNNGGEI TKFLLDHMSL 251 KDIDQAETFG QDLLNLGFLK YCNGVGNTFV NSKKFQYQWK NTAYMFANVP 301 MPGSEEPTTG ESLISRFNNW DGSSAKEIIQ SKIGNDQGAA KIQAPHISDN 351 ERTLFRMMDA LAASDKKYYQ ECFKMDALRC SVEELLIDHL SFMEKCESDR 401 LNAIKKATLD FCSTLGNKIS SLRLCIDKML TLENDIDPTA DLLQLLVKYK 451 TGSFKPQAIV YNNYYNPGSF QNFGVDLETR CRLDKKVVPL IISSIFSYMD 501 KIYPDLPNDK VRTSIWTDSV KLSLTHQLRN LLNKQQFHNE GEIFDILSTS 551 KLEPSTIASV VKIYLLELPD PLIPNDVSDI LRVLYLDYPP LVETALQNST 601 SSPENQQDDD NEEGFDTKRI RGLYTTLSSL SKPHIATLDA ITTHFYRLIK 651 ILKMGENGNE VADEFTVSIS QEFANCIIQS KITDDNEIGF KIFYDLLTHK 701 KQIFHELKRQ NSKN*

Amino Acid Sequence (or in FASTA format)

       1  MLSFCDYFWS EDLVSGLDVL FDRLYHGCEQ CDLFIQLFAS RMQFEVSHGR
      51  QLFGIEAGMD NLKAVQEDED EGVTVSRALR GILQEMSQEG THHLTIASNI
     101  ESLVLQPFSK WCIEHRERIQ YSEKTLLTNV NNFRKSKKYV GKLEKEYFNK
     151  CRQLEEFKRT HFNEDELANA MKSLKIQNKY EEDVAREKDH RFFNRIAGID
     201  FDYKTMKETL QLLLTKLPKT DYKLPLISYS LSNTNNGGEI TKFLLDHMSL
     251  KDIDQAETFG QDLLNLGFLK YCNGVGNTFV NSKKFQYQWK NTAYMFANVP
     301  MPGSEEPTTG ESLISRFNNW DGSSAKEIIQ SKIGNDQGAA KIQAPHISDN
     351  ERTLFRMMDA LAASDKKYYQ ECFKMDALRC SVEELLIDHL SFMEKCESDR
     401  LNAIKKATLD FCSTLGNKIS SLRLCIDKML TLENDIDPTA DLLQLLVKYK
     451  TGSFKPQAIV YNNYYNPGSF QNFGVDLETR CRLDKKVVPL IISSIFSYMD
     501  KIYPDLPNDK VRTSIWTDSV KLSLTHQLRN LLNKQQFHNE GEIFDILSTS
     551  KLEPSTIASV VKIYLLELPD PLIPNDVSDI LRVLYLDYPP LVETALQNST
     601  SSPENQQDDD NEEGFDTKRI RGLYTTLSSL SKPHIATLDA ITTHFYRLIK
     651  ILKMGENGNE VADEFTVSIS QEFANCIIQS KITDDNEIGF KIFYDLLTHK
     701  KQIFHELKRQ NSKN*

external links for Rgd2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rgd2p

1)	Roumanie O, et al. (2001) Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae. FEBS Lett 506(2):149-56
2)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41