VBA5/YKR105C Protein Information Help

Standard Name Vba5p 1
Systematic Name Ykr105cp
ORF Classification Uncharacterized
Description Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; VBA5 has a paralog, VBA3, that arose from a segmental duplication (1, 2, 3, 4, 5)
Name Description Vacuolar Basic Amino acid transporter 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 582
Molecular Weight (Da) 63,360
Isoelectric Point (pI) 8.32

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Vba5p (InterPro)
Transmembrane Domains There are 14 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 11 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B68B | P36172
MIPS: YKR105C
NCBI: 398365555 | 486615 | 549651 | NP_013031.3 | NM_001179895.3
GenBank/EMBL/DDBJ: DAA09256.1 | Z28330
External Classifications TC: 2.A.1.3 [The Drug:H+ Antiporter-2 (14 Spanner) (DHA2) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MEETKYSSQQ EIEGACGSDA SLNARGSNDS PMGLSLYLCL ASLTLVLFIT
      51  ALDILIVGTI IDVVAEQFGN YSKTGWLVTG YSLPNAILSL IWGRFASIIG
     101  FQHSLILAIL IFEAGSLIAA LASSMNMLIF GRVVAGVGGS GLQTLCFVIG
     151  CTMVGERSRP LVISILSCAF AVAAIVGPII GGAFTTHVTW RWCFYINLPI
     201  GGLAIIMFLL TYKAENKGIL QQIKDAIGTI SSFTFSKFRH QVNFKRLMNG
     251  IIFKFDFFGF ALCSAGLVLF LLGLTFGGNK YSWNSGQVIT YLVLGVLLFI
     301  FSLVYDFFLF DKFNPEPDNI SYRPLLLRRL VAKPAIIIVN MVTFLLCTGY
     351  NGQMIYSVQF FQLIFASSAW KAGLHLIPIV ITNVIAAIAS GVITKKLGLV
     401  KPLLIFGGVL GVIGAGLMTL MTNTSTKSTQ IGVLLLPGFS LGFALQASLM
     451  SAQLQITKDR PEAAMDFIEV TAFNTFMKSL GTTLGGVLST TVFSASFHNK
     501  VSRAHLEPYE GKTVDDMILY RLQNYDGSHS TIGNILSDSI KNVFWMDLGF
     551  YALGFLFCSF SSNKKLIIPK KDDTPEDNLE DK*

external links for Vba5p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome TCDB
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Vba5p
1) Shimazu M, et al.  (2005) A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae. J Biol Chem 280(6):4851-7
2) De Hertogh B, et al.  (2006) Emergence of species-specific transporters during evolution of the hemiascomycete phylum. Genetics 172(2):771-81
3) Gbelska Y, et al.  (2006) Evolution of gene families: the multidrug resistance transporter genes in five related yeast species. FEMS Yeast Res 6(3):345-55
4) Katju V, et al.  (2009) Variation in gene duplicates with low synonymous divergence in Saccharomyces cerevisiae relative to Caenorhabditis elegans. Genome Biol 10(7):R75
5) Shimazu M, et al.  (2012) Vba5p, a novel plasma membrane protein involved in amino acid uptake and drug sensitivity in Saccharomyces cerevisiae. Biosci Biotechnol Biochem 76(10):1993-5