SIR1/YKR101W Protein Information

Standard Name	Sir1p 1
Systematic Name	Ykr101wp
ORF Classification	Verified
Description	Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin (2, 3, 4)
Name Description	Silent Information Regulator 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	654
Molecular Weight (Da)	76,933
Isoelectric Point (pI)	9.41

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sir1p (InterPro)
Physical Interactions	There are 42 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000193281A \| P21691 MIPS: YKR101W NCBI: 134505 \| 157881139 \| 172606 \| 254763332 \| 398365529 \| 486607 \| 71042124 \| 71042125 \| 71042304 \| NP_013027.4 GenBank/EMBL/DDBJ: DAA09252.1 \| M38524 \| Z28326

Amino Acid Sequence (or in FASTA format)
1 MLQINSRLAV IDGWLVDTVK RKPINFRSPE VRLLLPNDDD YKKLSQQNLV 51 DWTRLKKDSN SVLVGVKSME LFKHIKLVLR EFFLLEDGRI ILKRIRSKLR 101 YKVVKKLTCK CCRLYLPKWG TVYIHPMLKD KEKPLAGVCE FSLDVNPDRE 151 YPLIEINVSH QYIIIEGFLL YLNERRLYRW NDNNLRSQVG LTKWAHLRKT 201 YNPVSLDILY SLNSNFYFVK DDLLFQLLGK RVFVKFCKVM ENGKCGKAPL 251 WYRVKRTTTA KATHIAYAIS NSTAPDSFKS KNNDYRFIVR EKPIVENTIS 301 NLDYSDIKKQ QFTEAEVVKR KISADISQIE NVHTQFNSQK EKNNIRVNKV 351 SSEVLDQISK FPVSRVTLLL MSAGQDKNYI ELVEELARRL EKICIEKTTQ 401 SLEEIRDTFQ ANPEMQASFD KEYYQSIEEY KITLELIKED LLITLIKQME 451 NMWAAEKKFS TEEEYVSPRF LVADGFLIDL AEEKPINPKD PRLLTLLKDH 501 QRAMIDQMNL VKWNDFKKYQ DPIPLKAKTL FKFCKQIKKK FLRGADFKLH 551 TLPTEANLKY EPERMTVLCS CVPILLDDQT VQYLYDDSII PEFEATSSYA 601 TKQSKCGRKM SLQMEPDLLF QEAIRRMRHL TAYDVLRRNY IAAFEELYMG 651 NCND*

Amino Acid Sequence (or in FASTA format)

       1  MLQINSRLAV IDGWLVDTVK RKPINFRSPE VRLLLPNDDD YKKLSQQNLV
      51  DWTRLKKDSN SVLVGVKSME LFKHIKLVLR EFFLLEDGRI ILKRIRSKLR
     101  YKVVKKLTCK CCRLYLPKWG TVYIHPMLKD KEKPLAGVCE FSLDVNPDRE
     151  YPLIEINVSH QYIIIEGFLL YLNERRLYRW NDNNLRSQVG LTKWAHLRKT
     201  YNPVSLDILY SLNSNFYFVK DDLLFQLLGK RVFVKFCKVM ENGKCGKAPL
     251  WYRVKRTTTA KATHIAYAIS NSTAPDSFKS KNNDYRFIVR EKPIVENTIS
     301  NLDYSDIKKQ QFTEAEVVKR KISADISQIE NVHTQFNSQK EKNNIRVNKV
     351  SSEVLDQISK FPVSRVTLLL MSAGQDKNYI ELVEELARRL EKICIEKTTQ
     401  SLEEIRDTFQ ANPEMQASFD KEYYQSIEEY KITLELIKED LLITLIKQME
     451  NMWAAEKKFS TEEEYVSPRF LVADGFLIDL AEEKPINPKD PRLLTLLKDH
     501  QRAMIDQMNL VKWNDFKKYQ DPIPLKAKTL FKFCKQIKKK FLRGADFKLH
     551  TLPTEANLKY EPERMTVLCS CVPILLDDQT VQYLYDDSII PEFEATSSYA
     601  TKQSKCGRKM SLQMEPDLLF QEAIRRMRHL TAYDVLRRNY IAAFEELYMG
     651  NCND*

external links for Sir1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Sir1p

1)	Rine J, et al. (1979) A suppressor of mating-type locus mutations in Saccharomyces cerevisiae: evidence for and identification of cryptic mating-type loci. Genetics 93(4):877-901
2)	Rine J and Herskowitz I (1987) Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae. Genetics 116(1):9-22
3)	Sharp JA, et al. (2003) The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin. Genes Dev 17(19):2356-61
4)	Bose ME, et al. (2004) The origin recognition complex and Sir4 protein recruit Sir1p to yeast silent chromatin through independent interactions requiring a common Sir1p domain. Mol Cell Biol 24(2):774-86