PCK1/YKR097W Protein Information Help

Standard Name Pck1p
Systematic Name Ykr097wp
Alias Jpm2p , Ppc1p
ORF Classification Verified
Description Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Phosphoenolpyruvate CarboxyKinase 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 549
Molecular Weight (Da) 60,983
Isoelectric Point (pI) 6.31

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pck1p (InterPro)
Physical Interactions There are 24 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000004F940 | P10963
MIPS: YKR097W
NCBI: 1000080 | 398365505 | 4126 | 486591 | 51830446 | 548582 | NP_013023.3 | NM_001179887.3
GenBank/EMBL/DDBJ: DAA09248.1 | AY723843 | U24234 | X13096 | Z28322
External Classifications EC: 4.1.1.49 [Phosphoenolpyruvate carboxykinase (ATP)]
Amino Acid Sequence (or in FASTA format)
 
       1  MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN
      51  KTVISSSGAL IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT
     101  WSINRERAAD YLRTRDHIYI VDAFAGWDPK YRIKVRVVCA RAYHALFMTN
     151  MLIRPTEEEL AHFGEPDFTV WNAGQFPANL HTQDMSSKST IEINFKAMEM
     201  IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI QNGDVTLFFG
     251  LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP
     301  EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP
     351  CLADSHPKNI ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG
     401  VTEPEPTFSS CFGQPFLALH PIRYATMLAT KMSQHKANAY LINTGWTGSS
     451  YVSGGKRCPL KYTRAILDSI HDGSLANETY ETLPIFNLQV PTKVNGVPAE
     501  LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD VLAAGPQFE*

external links for Pck1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB GeneMANIA

YOGY


References cited on this page View Complete Literature Guide for Pck1p
1) Haurie V, et al.  (2001) The transcriptional activator Cat8p provides a major contribution to the reprogramming of carbon metabolism during the diauxic shift in Saccharomyces cerevisiae. J Biol Chem 276(1):76-85
2) Yin Z, et al.  (2000) Differential post-transcriptional regulation of yeast mRNAs in response to high and low glucose concentrations. Mol Microbiol 35(3):553-65
3) Kuo MH and Grayhack E  (1994) A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism. Mol Cell Biol 14(1):348-59
4) Proft M, et al.  (1995) Identification and characterization of regulatory elements in the phosphoenolpyruvate carboxykinase gene PCK1 of Saccharomyces cerevisiae. Mol Gen Genet 246(3):367-73
5) Valdes-Hevia MD, et al.  (1989) Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae. FEBS Lett 258(2):313-6
6) Haarasilta S and Taskinen L  (1977) Location of three key enzymes of gluconeogenesis in baker's yeast. Arch Microbiol 113(1-2):159-61
7) Haarasilta S and Oura E  (1975) On the activity and regulation of anaplerotic and gluconeogenetic enzymes during the growth process of baker's yeast. The biphasic growth. Eur J Biochem 52(1):1-7
8) Llanos L, et al.  (2001) Mutation Arg336 to Lys in Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase originates an enzyme with increased oxaloacetate decarboxylase activity. FEBS Lett 493(1):1-5