PRP16/YKR086W Protein Information

Standard Name	Prp16p 1
Systematic Name	Ykr086wp
Alias	Rna16p 2 , Prp23p 3, 4
ORF Classification	Verified
Description	DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step (5, 6, 7)
Name Description	Pre-mRNA Processing 4

Experimental Data
Molecules/cell	1250 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,071
Molecular Weight (Da)	121,651
Isoelectric Point (pI)	8.25

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prp16p (InterPro)
Physical Interactions	There are 44 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000132144 \| P15938 MIPS: YKR086W NCBI: 172270 \| 398365421 \| 415906 \| 486571 \| 548590 \| NP_013012.3 \| NM_001179876.3 GenBank/EMBL/DDBJ: DAA09236.1 \| M31524 \| Z27116 \| Z28311
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.13 [RNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MGHSGREERI KDIFKELTSK ELTPGLLLTL QKLAQKPNTN LEQFIASCKA 51 LTKLSSNNPI IFNELLELLK NKSEEDSTGP KKIAPSINKR KKFKIQLDLD 101 DNEDELDSPV QKKPAPTRTL FKRIDKLKAK QLRQYSPTVK DPSPNSEQQT 151 QNGHAETKDY EPTRSEVVEE DREWYDNDDD YGNLVPEPLS ELPEEAKLLP 201 VIRNIDNDDA LRNTVQLYPI PLKQRMEWIP PFLSKFALEN KVPTSIIIGS 251 ISETSSQVSA LSMVNPFRNP DSEFSANAKR GSKLVALRRI NMEHIQQSRD 301 NTTVLNTAMG EVLGLENNNK AKDKSNQKIC DDTALFTPSK DDIKHTKEQL 351 PVFRCRSQLL SLIRENQVVV IIGETGSGKT TQLAQYLYEE GYANDRGKSI 401 VVTQPRRVAA ISVAKRVAME MQVPLGKEVG YSIRFEDVTD SECTKLKFVT 451 DGILLRETLL DDTLDKYSCV IIDEAHERSL NTDILLGFFK ILLARRRDLK 501 LIITSATMNA KKFSAFFGNA PQFTIPGRTF PVQTIYTSNP VQDYVEAAVS 551 QAVKIHLAND CSSGDILIFM TGQEDIETTF DTLQEKFLQV YSKKFGTANF 601 EEINDIEILP IYSALPADLQ FKIFQDLHGT KRKIIIATNI AETSLTIKGI 651 RYVIDCGYSK LKVYNPKIGL DSLVITPISK ANADQRSGRA GRTAPGTAYR 701 LYTEDTFKED MYLQTIPEIQ RTNLSNTLLL LKSLDVTDEL SKFPFIDKPP 751 LQTFLSSLYE LWFIGAIDTS GQLTPLGLQM AKFPLQPSLS KILLIAVRNG 801 CSDEMLTIVS MLSVPQVFYR PKERQKEADI ARNKFFIAKS DHLTLLNVFE 851 QWRANNFSSH WCNKHFVQYK SLVRARDIRD QLLTILKSQK IPVISSGKDW 901 DIIKKCICSG FAHQAAKITG LRNYVHLKTG VSVQLHPTSA LHGLGDLPPY 951 VVYHELLMTS KEYICCVTSV DPFWLMEYGG LLYDIKRIKN DQEATTTGLF 1001 GEHYEHTLDK VEDDIDINIR RCKDMRDSVI QELKMTDNSN KEDKKQKTKK 1051 QNILNGKENS MKPFKRRKPF F*

Amino Acid Sequence (or in FASTA format)

       1  MGHSGREERI KDIFKELTSK ELTPGLLLTL QKLAQKPNTN LEQFIASCKA
      51  LTKLSSNNPI IFNELLELLK NKSEEDSTGP KKIAPSINKR KKFKIQLDLD
     101  DNEDELDSPV QKKPAPTRTL FKRIDKLKAK QLRQYSPTVK DPSPNSEQQT
     151  QNGHAETKDY EPTRSEVVEE DREWYDNDDD YGNLVPEPLS ELPEEAKLLP
     201  VIRNIDNDDA LRNTVQLYPI PLKQRMEWIP PFLSKFALEN KVPTSIIIGS
     251  ISETSSQVSA LSMVNPFRNP DSEFSANAKR GSKLVALRRI NMEHIQQSRD
     301  NTTVLNTAMG EVLGLENNNK AKDKSNQKIC DDTALFTPSK DDIKHTKEQL
     351  PVFRCRSQLL SLIRENQVVV IIGETGSGKT TQLAQYLYEE GYANDRGKSI
     401  VVTQPRRVAA ISVAKRVAME MQVPLGKEVG YSIRFEDVTD SECTKLKFVT
     451  DGILLRETLL DDTLDKYSCV IIDEAHERSL NTDILLGFFK ILLARRRDLK
     501  LIITSATMNA KKFSAFFGNA PQFTIPGRTF PVQTIYTSNP VQDYVEAAVS
     551  QAVKIHLAND CSSGDILIFM TGQEDIETTF DTLQEKFLQV YSKKFGTANF
     601  EEINDIEILP IYSALPADLQ FKIFQDLHGT KRKIIIATNI AETSLTIKGI
     651  RYVIDCGYSK LKVYNPKIGL DSLVITPISK ANADQRSGRA GRTAPGTAYR
     701  LYTEDTFKED MYLQTIPEIQ RTNLSNTLLL LKSLDVTDEL SKFPFIDKPP
     751  LQTFLSSLYE LWFIGAIDTS GQLTPLGLQM AKFPLQPSLS KILLIAVRNG
     801  CSDEMLTIVS MLSVPQVFYR PKERQKEADI ARNKFFIAKS DHLTLLNVFE
     851  QWRANNFSSH WCNKHFVQYK SLVRARDIRD QLLTILKSQK IPVISSGKDW
     901  DIIKKCICSG FAHQAAKITG LRNYVHLKTG VSVQLHPTSA LHGLGDLPPY
     951  VVYHELLMTS KEYICCVTSV DPFWLMEYGG LLYDIKRIKN DQEATTTGLF
    1001  GEHYEHTLDK VEDDIDINIR RCKDMRDSVI QELKMTDNSN KEDKKQKTKK
    1051  QNILNGKENS MKPFKRRKPF F*

external links for Prp16p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Prp16p

1)	Burgess S, et al. (1990) A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. Cell 60(5):705-17
2)	Couto JR, et al. (1987) A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation. Genes Dev 1(5):445-55
3)	Schwer B and Guthrie C (1991) PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349(6309):494-9
4)	Vijayraghavan U, et al. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
5)	Wang Y, et al. (1998) The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro. Curr Biol 8(8):441-51
6)	Umen JG and Guthrie C (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85
7)	Tseng CK, et al. (2011) DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps. RNA 17(1):145-54
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41