HBS1/YKR084C Protein Information Help

Standard Name Hbs1p
Systematic Name Ykr084cp
ORF Classification Verified
Description GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Hsp70 subfamily B Suppressor 1
Experimental Data
Molecules/cell 2750 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 611
Molecular Weight (Da) 68,729
Isoelectric Point (pI) 5.09

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hbs1p (InterPro)
Physical Interactions There are 16 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012C32A | P32769
MIPS: YKR084C
NCBI: 312208136 | 312208137 | 312208138 | 312208139 | 334359142 | 398365409 | 415904 | 486567 | 544478 | NP_013010.3
GenBank/EMBL/DDBJ: DAA09234.1 | M98437 | Z27116 | Z28309
Amino Acid Sequence (or in FASTA format)
 
       1  MAYSDYSDGA DDMPDFHDEG EFDDYLNDDE YDLMNEVFPT LKAQLQDYQG
      51  WDNLSLKLAL FDNNFDLEST LAELKKTLKK KKTPKKPIAA ANGSANVTQK
     101  LANISISQQR PNDRLPDWLD EEESEGERNG EEANDEKTVQ RYYKTTVPTK
     151  PKKPHDISAF VKSALPHLSF VVLGHVDAGK STLMGRLLYD LNIVNQSQLR
     201  KLQRESETMG KSSFKFAWIM DQTNEERERG VTVSICTSHF STHRANFTIV
     251  DAPGHRDFVP NAIMGISQAD MAILCVDCST NAFESGFDLD GQTKEHMLLA
     301  SSLGIHNLII AMNKMDNVDW SQQRFEEIKS KLLPYLVDIG FFEDNINWVP
     351  ISGFSGEGVY KIEYTDEVRQ WYNGPNLMST LENAAFKISK ENEGINKDDP
     401  FLFSVLEIIP SKKTSNDLAL VSGKLESGSI QPGESLTIYP SEQSCIVDKI
     451  QVGSQQGQST NHEETDVAIK GDFVTLKLRK AYPEDIQNGD LAASVDYSSI
     501  HSAQCFVLEL TTFDMNRPLL PGTPFILFIG VKEQPARIKR LISFIDKGNT
     551  ASKKKIRHLG SKQRAFVEIE LIEVKRWIPL LTAHENDRLG RVVLRKDGRT
     601  IAAGKISEIT Q*

external links for Hbs1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Hbs1p
1) Nelson RJ, et al.  (1992) The translation machinery and 70 kd heat shock protein cooperate in protein synthesis. Cell 71(1):97-105
2) Carr-Schmid A, et al.  (2002) Novel G-protein complex whose requirement is linked to the translational status of the cell. Mol Cell Biol 22(8):2564-74
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Doma MK and Parker R  (2006) Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation. Nature 440(7083):561-4
5) Passos DO, et al.  (2009) Analysis of Dom34 and its function in no-go decay. Mol Biol Cell 20(13):3025-32
6) Shoemaker CJ, et al.  (2010) Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. Science 330(6002):369-72
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41