MTD1/YKR080W Protein Information

Standard Name	Mtd1p 1
Systematic Name	Ykr080wp
ORF Classification	Verified
Description	NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline (2, 3, 4)
Name Description	Methylene Tetrahydrafolate Dehydrogenase 1

Experimental Data
Molecules/cell	16200 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	320
Molecular Weight (Da)	36,239
Isoelectric Point (pI)	6.98

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mtd1p (InterPro)
Physical Interactions	There are 10 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000111296 \| Q02046 MIPS: YKR080W NCBI: 12084632 \| 12084633 \| 172016 \| 398365387 \| 415900 \| 417321 \| 486559 \| NP_013006.3 GenBank/EMBL/DDBJ: DAA09230.1 \| L02934 \| Z27116 \| Z28305
External Classifications	EC: 1.5.1.15 [Methylenetetrahydrofolate dehydrogenase (NAD(+))]

Amino Acid Sequence (or in FASTA format)
1 MSKPGRTILA SKVAETFNTE IINNVEEYKK THNGQGPLLV GFLANNDPAA 51 KMYATWTQKT SESMGFRYDL RVIEDKDFLE EAIIQANGDD SVNGIMVYFP 101 VFGNAQDQYL QQVVCKEKDV EGLNHVYYQN LYHNVRYLDK ENRLKSILPC 151 TPLAIVKILE FLKIYNNLLP EGNRLYGKKC IVINRSEIVG RPLAALLAND 201 GATVYSVDVN NIQKFTRGES LKLNKHHVED LGEYSEDLLK KCSLDSDVVI 251 TGVPSENYKF PTEYIKEGAV CINFACTKNF SDDVKEKASL YVPMTGKVTI 301 AMLLRNMLRL VRNVELSKEK *

Amino Acid Sequence (or in FASTA format)

       1  MSKPGRTILA SKVAETFNTE IINNVEEYKK THNGQGPLLV GFLANNDPAA
      51  KMYATWTQKT SESMGFRYDL RVIEDKDFLE EAIIQANGDD SVNGIMVYFP
     101  VFGNAQDQYL QQVVCKEKDV EGLNHVYYQN LYHNVRYLDK ENRLKSILPC
     151  TPLAIVKILE FLKIYNNLLP EGNRLYGKKC IVINRSEIVG RPLAALLAND
     201  GATVYSVDVN NIQKFTRGES LKLNKHHVED LGEYSEDLLK KCSLDSDVVI
     251  TGVPSENYKF PTEYIKEGAV CINFACTKNF SDDVKEKASL YVPMTGKVTI
     301  AMLLRNMLRL VRNVELSKEK *

external links for Mtd1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Mtd1p

1)	West MG, et al. (1993) Cloning and characterization of the Saccharomyces cerevisiae gene encoding NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase. J Biol Chem 268(1):153-60
2)	West MG, et al. (1996) Metabolic role of cytoplasmic isozymes of 5,10-methylenetetrahydrofolate dehydrogenase in Saccharomyces cerevisiae. Biochemistry 35(9):3122-32
3)	Denis V and Daignan-Fornier B (1998) Synthesis of glutamine, glycine and 10-formyl tetrahydrofolate is coregulated with purine biosynthesis in Saccharomyces cerevisiae. Mol Gen Genet 259(3):246-55
4)	Santiago TC and Mamoun CB (2003) Genome expression analysis in yeast reveals novel transcriptional regulation by inositol and choline and new regulatory functions for Opi1p, Ino2p, and Ino4p. J Biol Chem 278(40):38723-30
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41