ECM4/YKR076W Protein Information

Standard Name	Ecm4p 1
Systematic Name	Ykr076wp
Alias	Gto2p 2
ORF Classification	Verified
Description	Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm (1, 2, 3, 4)
Name Description	ExtraCellular Mutant 1

Experimental Data
Molecules/cell	1670 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	370
Molecular Weight (Da)	43,273
Isoelectric Point (pI)	6.89

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ecm4p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F93E \| P36156 MIPS: YKR076W NCBI: 398365369 \| 486551 \| 549634 \| NP_013002.3 GenBank/EMBL/DDBJ: DAA09226.1 \| Z28301
External Classifications	EC: 1.8.5.1 [Glutathione dehydrogenase (ascorbate)] EC: 2.5.1.18 [Glutathione transferase]

Amino Acid Sequence (or in FASTA format)
1 MSKQWASGTN GAFKRQVSSF RETISKQHPI YKPAKGRYWL YVSLACPWAH 51 RTLITRALKG LTSVIGCSVV HWHLDEKGWR FLDMEKQLED SEDFLEHWHD 101 VAGGIRTAKE DSSKSFAEIK NDSQRFMVDA TNEPHYGYKR ISDLYYKSDP 151 QYSARFTVPV LWDLETQTIV NNESSEIIRI LNSSAFDEFV DDDHKKTDLV 201 PAQLKTQIDD FNSWVYDSIN NGVYKTGFAE KAEVYESEVN NVFEHLDKVE 251 KILSDKYSKL KAKYGEEDRQ KILGEFFTVG DQLTEADIRL YTTVIRFDPV 301 YVQHFKCNFT SIRAGYPFIH LWVRNLYWNY DAFRYTTDFD HIKLHYTRSH 351 TRINPLGITP LGPKPDIRPL *

Amino Acid Sequence (or in FASTA format)

       1  MSKQWASGTN GAFKRQVSSF RETISKQHPI YKPAKGRYWL YVSLACPWAH
      51  RTLITRALKG LTSVIGCSVV HWHLDEKGWR FLDMEKQLED SEDFLEHWHD
     101  VAGGIRTAKE DSSKSFAEIK NDSQRFMVDA TNEPHYGYKR ISDLYYKSDP
     151  QYSARFTVPV LWDLETQTIV NNESSEIIRI LNSSAFDEFV DDDHKKTDLV
     201  PAQLKTQIDD FNSWVYDSIN NGVYKTGFAE KAEVYESEVN NVFEHLDKVE
     251  KILSDKYSKL KAKYGEEDRQ KILGEFFTVG DQLTEADIRL YTTVIRFDPV
     301  YVQHFKCNFT SIRAGYPFIH LWVRNLYWNY DAFRYTTDFD HIKLHYTRSH
     351  TRINPLGITP LGPKPDIRPL *

external links for Ecm4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Ecm4p

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Herrero E (2005) Evolutionary relationships between Saccharomyces cerevisiae and other fungal species as determined from genome comparisons. Rev Iberoam Micol 22(4):217-22
3)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4)	Garcera A, et al. (2006) Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases. Biochem J 398(2):187-96
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41