SIS2/YKR072C Protein Information Help

Standard Name Sis2p 1, 2
Systematic Name Ykr072cp
Alias Hal3p 3
ORF Classification Verified
Description Negative regulatory subunit of protein phosphatase 1 (Ppz1p); also a subunit of phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; SIS2 has a paralog, VHS3, that arose from the whole genome duplication (4, 5, 6, 7)
Name Description SIt4 Suppressor 1
Experimental Data
Molecules/cell 3750 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 562
Molecular Weight (Da) 62,478
Isoelectric Point (pI) 4.4

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sis2p (InterPro)
Physical Interactions There are 30 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001359B5 | P36024
MIPS: YKR072C
NCBI: 430984 | 486545 | 548925 | 6322925 | NP_012998.1
GenBank/EMBL/DDBJ: DAA09223.1 | U01878 | Z28297
Amino Acid Sequence (or in FASTA format)
 
       1  MTAVASTSGK QDADHNQSIE CPRFSRGQKE ILLDHEDAKG KDSIINSPVS
      51  GRQSISPTLS NATTTTTKSI MNATGTSGAV VSNTPEPGLK RVPAVTFSDL
     101  KQQQKQDSLT QLKNDSERTK SPNSNPAPVS NSIPGNHAVI PNHTNTSRTT
     151  QLSGSPLVNE MKDYDPKKKD SALKIVDTMK PDKIMATSTP ISRENNKVTA
     201  KAPTSITLRK EDAQDQANNV SGQINVRSTP EETPVKQSVI PSIIPKRENS
     251  KNLDPRLPQD DGKLHVLFGA TGSLSVFKIK PMIKKLEEIY GRDRISIQVI
     301  LTQSATQFFE QRYTKKIIKS SEKLNKMSQY ESTPATPVTP TPGQCNMAQV
     351  VELPPHIQLW TDQDEWDAWK QRTDPVLHIE LRRWADILVV APLTANTLSK
     401  IALGLCDNLL TSVIRAWNPS YPILLAPSMV SSTFNSMMTK KQLQTIKEEM
     451  SWVTVFKPSE KVMDINGDIG LGGMMDWNEI VNKIVMKLGG YPKNNEEEDD
     501  DEDEEEDDDE EEDTEDKNEN NNDDDDDDDD DDDDDDDDDD DDDDDDEDED
     551  EAETPGIIDK HQ*

external links for Sis2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Sis2p
1) Di Como CJ, et al.  (1995) Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants. Genetics 139(1):95-107
2) Sutton, A. and Arndt, K.  (1992) Personal Communication, Mortimer Map Edition 11
3) Ferrando A, et al.  (1995) Regulation of cation transport in Saccharomyces cerevisiae by the salt tolerance gene HAL3. Mol Cell Biol 15(10):5470-81
4) de Nadal E, et al.  (1998) The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase. Proc Natl Acad Sci U S A 95(13):7357-62
5) Yenush L, et al.  (2002) The Ppz protein phosphatases are key regulators of K+ and pH homeostasis: implications for salt tolerance, cell wall integrity and cell cycle progression. EMBO J 21(5):920-9
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Ruiz A, et al.  (2009) Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis. Nat Chem Biol 5(12):920-8
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41