MET1/YKR069W Protein Information Help

Standard Name Met1p 1, 2
Systematic Name Ykr069wp
Alias Met20p 1, 2, 3
ORF Classification Verified
Description S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis (1, 4, 5)
Name Description METhionine requiring 2
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 593
Molecular Weight (Da) 66,125
Isoelectric Point (pI) 6.53

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Met1p (InterPro)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 3 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013621A | P36150
MIPS: YKR069W
NCBI: 398365329 | 486539 | 549009 | NP_012995.3
GenBank/EMBL/DDBJ: DAA09220.1 | Z28294
External Classifications EC: 2.1.1.107 [Uroporphyrinogen-III C-methyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MVRDLVTLPS SLPLITAGFA TDQVHLLIGT GSTDSVSVCK NRIHSILNAG
      51  GNPIVVNPSS PSHTKQLQLE FGKFAKFEIV EREFRLSDLT TLGRVLVCKV
     101  VDRVFVDLPI TQSRLCEEIF WQCQKLRIPI NTFHKPEFST FNMIPTWVDP
     151  KGSGLQISVT TNGNGYILAN RIKRDIISHL PPNISEVVIN MGYLKDRIIN
     201  EDHKALLEEK YYQTDMSLPG FGYGLDEDGW ESHKFNKLIR EFEMTSREQR
     251  LKRTRWLSQI MEYYPMNKLS DIKLEDFETS SSPNKKTKQE TVTEGVVPPT
     301  DENIENGTKQ LQLSEVKKEE GPKKLGKISL VGSGPGSVSM LTIGALQEIK
     351  SADIILADKL VPQAILDLIP PKTETFIAKK FPGNAERAQQ ELLAKGLESL
     401  DNGLKVVRLK QGDPYIFGRG GEEFNFFKDH GYIPVVLPGI SSSLACTVLA
     451  QIPATQRDIA DQVLICTGTG RKGALPIIPE FVESRTTVFL MALHRANVLI
     501  TGLLKHGWDG DVPAAIVERG SCPDQRVTRT LLKWVPEVVE EIGSRPPGVL
     551  VVGKAVNALV EKDLINFDES RKFVIDEGFR EFEVDVDSLF KLY*

external links for Met1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Met1p
1) Hansen J, et al.  (1997) Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes. FEBS Lett 401(1):20-4
2) Masselot M and De Robichon-Szulmajster H  (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
3) Masselot M and Surdin-Kerjan Y  (1977) Methionine biosynthesis in Saccharomyces cerevisiae. II. Gene-enzyme relationships in the sulfate assimilation pathway. Mol Gen Genet 154(1):23-30
4) Raux E, et al.  (1999) The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis. Biochem J 338 ( Pt 3)():701-8
5) Thomas D, et al.  (1992) Physiological analysis of mutants of Saccharomyces cerevisiae impaired in sulphate assimilation. J Gen Microbiol 138(10):2021-8