OAF3/YKR064W Protein Information Help

Standard Name Oaf3p 1
Systematic Name Ykr064wp
ORF Classification Verified
Description Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description Oleate Activated transcription Factor 1
Experimental Data
Molecules/cell 149 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 863
Molecular Weight (Da) 101,824
Isoelectric Point (pI) 6.84

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Oaf3p (InterPro)
Physical Interactions There are 5 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B654 | P36023
MIPS: YKR064W
NCBI: 486531 | 549625 | 6322917 | NP_012990.1
GenBank/EMBL/DDBJ: DAA09215.1 | Z28289
Amino Acid Sequence (or in FASTA format)
 
       1  MGYDSQVRTK KRHRITVVCT NCKKRKSKCD RTKPCGTCVR LGDVDSCVYL
      51  TDSSGQPESS PSLNDADPLR KQSTPAERIS PGFIKKRRSS QTRQDEDHWQ
     101  RVRELENQSS LYYLPIHEET PFFIDLIPNG FYLETKRSAD NLFGLFTDRA
     151  IENRDPYLQA MVTFRSIAIK KMMDKLGSNG NNVKNGSLPK SFEALSTFDA
     201  DDERHISDDV VGKGNNFRMH QTIHKSLFNK FAQYRENNAK KFSSETILAK
     251  DYLPPLKILE SEVLALFEEK IYNMIPIFDM KVLRHEITIF YQNIVEKGNP
     301  ISIKHYDHMV FCIILLIIKI CRLSVQFSKL TPYIYPVLQE IDTSKFLALV
     351  KHYLFETKVL RKCNLLQLQC LILLRFLHWC APEDGDGPET QYCQILMGTI
     401  ISSCKEMGIN WYCFSHPEKY SFKINRHTRP SYDIMKPSDY ISVFRKIWSY
     451  VLFWDRKMCF ISGEECQIGK TLQCHFKEEA DTPTWYIRML TLDNLMKKIN
     501  DTLNDDPGKV DLNLLHRLIN DLKRNFHILK SLSKNEKETM RHFDFEMEWI
     551  IDLFSLSLLH GEMIFYEYDC NITKFYKSFQ DLWDMVIHIS EKCYNYFFNS
     601  DALEVDSLTK FYTNRIVEIV ANKVLVIVPA FILRGDRFKT IQYADKKKMI
     651  EFLYGVSSVY FNEFGFEYYR CFRKMFTAKI AYKILNRSCE KDAWRIILKF
     701  LLNELKLEDN GDSYIDYNDM RLNDICPIIL EFQETVQKYD GYRPDILSIW
     751  NNEFYPIGKY NDDMTGFKFQ MRIKEMQEFL DMEKYSDRFN IFSSFYDHAS
     801  SQLAKHTEVD TNISITNEQV AEIPQKELLQ QPLAPALPVN DLIVSEFDVI
     851  EDIFDPVDFV SFF*

external links for Oaf3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Oaf3p
1) Smith JJ, et al.  (2007) Transcriptional responses to fatty acid are coordinated by combinatorial control. Mol Syst Biol 3():115
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
4) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
5) MacPherson S, et al.  (2006) A fungal family of transcriptional regulators: the zinc cluster proteins. Microbiol Mol Biol Rev 70(3):583-604
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41