TIF1/YKR059W Protein Information Help

Standard Name Tif1p 1, 2
Systematic Name Ykr059wp
ORF Classification Verified
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication (3, 4, 5, 6, 7, 8)
Name Description Translation Initiation Factor 1
Gene Product eIF4A
Experimental Data
Molecules/cell 106000 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 395
Molecular Weight (Da) 44,697
Isoelectric Point (pI) 4.85

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tif1p (InterPro)
Physical Interactions There are 73 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000004F92D | P10081
MIPS: YKR059W
NCBI: 1015544 | 11513342 | 11513344 | 1217992 | 124218 | 398365253 | 4621 | 4623 | 4832 | 486521 | 6322323 | 6730574 | 854559 | 8569479 | NP_012397.1 | NP_012985.3 | NM_001179849.3 | NM_001181571.1
GenBank/EMBL/DDBJ: AAA91645.1 | CAA31301.1 | CAA31302.1 | CAA41110.1 | CAA60817.1 | CAA82138.1 | CAA89433.1 | DAA08662.1 | DAA09210.1 | U25436 | X12813 | X12814 | X58099 | X87371 | Z28284 | Z49413
External Classifications EC: 3.6.4.13 [RNA helicase]
Amino Acid Sequence (or in FASTA format)
       1  MSEGITDIEE SQIQTNYDKV VYKFDDMELD ENLLRGVFGY GFEEPSAIQQ
      51  RAIMPIIEGH DVLAQAQSGT GKTGTFSIAA LQRIDTSVKA PQALMLAPTR
     101  ELALQIQKVV MALAFHMDIK VHACIGGTSF VEDAEGLRDA QIVVGTPGRV
     151  FDNIQRRRFR TDKIKMFILD EADEMLSSGF KEQIYQIFTL LPPTTQVVLL
     201  SATMPNDVLE VTTKFMRNPV RILVKKDELT LEGIKQFYVN VEEEEYKYEC
     251  LTDLYDSISV TQAVIFCNTR RKVEELTTKL RNDKFTVSAI YSDLPQQERD
     301  TIMKEFRSGS SRILISTDLL ARGIDVQQVS LVINYDLPAN KENYIHRIGR
     351  GGRFGRKGVA INFVTNEDVG AMRELEKFYS TQIEELPSDI ATLLN*    

external links for Tif1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
YGOB CYC2008 (complexes) MIPS YPL+
YOGY Complexome Pfam domains YeastGFP

DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository

GeneMANIA


IMP


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Tif1p
1) Linder P and Slonimski PP  (1989) An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation. Proc Natl Acad Sci U S A 86(7):2286-90
2) Mueller, P. and Linder, P.  (1989) Personal Communication, Mortimer Map Edition 10
3) Blum S, et al.  (1989) Translation in Saccharomyces cerevisiae: initiation factor 4A-dependent cell-free system. Proc Natl Acad Sci U S A 86(16):6043-6
4) Neff CL and Sachs AB  (1999) Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally. Mol Cell Biol 19(8):5557-64
5) Chang TH, et al.  (1990) Identification of five putative yeast RNA helicase genes. Proc Natl Acad Sci U S A 87(4):1571-5
6) Caruthers JM, et al.  (2000) Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase. Proc Natl Acad Sci U S A 97(24):13080-5
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41