TRM2/YKR056W Protein Information

Standard Name	Trm2p
Systematic Name	Ykr056wp
Alias	Nuc2p , Nud1p , Rnc1p
ORF Classification	Verified
Description	tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair (1, 2, 3)
Name Description	Transfer RNA Methyltransferase

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	639
Molecular Weight (Da)	72,852
Isoelectric Point (pI)	9.27

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Trm2p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000034F5D2 \| P33753 MIPS: YKR056W NCBI: 110825750 \| 30267784 \| 397360 \| 42759862 \| 4355 \| 464650 \| 486515 \| 9755890 \| NP_012982.2 GenBank/EMBL/DDBJ: DAA09207.1 \| AJ250970 \| AY260897 \| Z14126 \| Z25734 \| Z28281
External Classifications	EC: 2.1.1.35 [tRNA (uracil-5-)-methyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MYEQFEFSFF FFENSDNKVK YKAHLISSIK RWSIITCMRC FWTVQKSIFK 51 ARFFACRNFV KKHNYKLIST MTGSTEMVPP TMKHTVDNKR LSSPLTDSGN 101 RRTKKPKLRK YKAKKVETTS PMGVLEFEVN DLLKSQNLSR EQVLNDVTSI 151 LNDKSSTDGP IVLQYHREVK NVKVLEITSN GNGLALIDNP VETEKKQVVI 201 IPFGLPGDVV NIKVFKTHPY YVESDLLDVV EKSPMRRDDL IRDKYFGKSS 251 GSQLEFLTYD DQLELKRKTI MNAYKFFAPR LVAEKLLPPF DTTVASPLQF 301 GYRTKITPHF DMPKRKQKEL SVRPPLGFGQ KGRPQWRKDT LDIGGHGSIL 351 DIDECVLATE VLNKGLTNER RKFEQEFKNY KKGATILLRE NTTILDPSKP 401 TLEQLTEEAS RDENGDISYV EVEDKKNNVR LAKTCVTNPR QIVTEYVDGY 451 TFNFSAGEFF QNNNSILPIV TKYVRDNLQA PAKGDDNKTK FLVDAYCGSG 501 LFSICSSKGV DKVIGVEISA DSVSFAEKNA KANGVENCRF IVGKAEKLFE 551 SIDTPSENTS VILDPPRKGC DELFLKQLAA YNPAKIIYIS CNVHSQARDV 601 EYFLKETENG SAHQIESIRG FDFFPQTHHV ESVCIMKRI*

Amino Acid Sequence (or in FASTA format)

       1  MYEQFEFSFF FFENSDNKVK YKAHLISSIK RWSIITCMRC FWTVQKSIFK
      51  ARFFACRNFV KKHNYKLIST MTGSTEMVPP TMKHTVDNKR LSSPLTDSGN
     101  RRTKKPKLRK YKAKKVETTS PMGVLEFEVN DLLKSQNLSR EQVLNDVTSI
     151  LNDKSSTDGP IVLQYHREVK NVKVLEITSN GNGLALIDNP VETEKKQVVI
     201  IPFGLPGDVV NIKVFKTHPY YVESDLLDVV EKSPMRRDDL IRDKYFGKSS
     251  GSQLEFLTYD DQLELKRKTI MNAYKFFAPR LVAEKLLPPF DTTVASPLQF
     301  GYRTKITPHF DMPKRKQKEL SVRPPLGFGQ KGRPQWRKDT LDIGGHGSIL
     351  DIDECVLATE VLNKGLTNER RKFEQEFKNY KKGATILLRE NTTILDPSKP
     401  TLEQLTEEAS RDENGDISYV EVEDKKNNVR LAKTCVTNPR QIVTEYVDGY
     451  TFNFSAGEFF QNNNSILPIV TKYVRDNLQA PAKGDDNKTK FLVDAYCGSG
     501  LFSICSSKGV DKVIGVEISA DSVSFAEKNA KANGVENCRF IVGKAEKLFE
     551  SIDTPSENTS VILDPPRKGC DELFLKQLAA YNPAKIIYIS CNVHSQARDV
     601  EYFLKETENG SAHQIESIRG FDFFPQTHHV ESVCIMKRI*

external links for Trm2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Trm2p

1)	Nordlund ME, et al. (2000) Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae. RNA 6(6):844-60
2)	Johansson MJ and Bystrom AS (2002) Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation. RNA 8(3):324-35
3)	Choudhury SA, et al. (2007) Functional and genetic analysis of the Saccharomyces cerevisiae RNC1/TRM2: evidences for its involvement in DNA double-strand break repair. Mol Cell Biochem 300(1-2):215-26