KAE1/YKR038C Protein Information

Standard Name	Kae1p 1
Systematic Name	Ykr038cp
ORF Classification	Verified
Description	Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription (1, 2, 3, 4, 5, 6)
Name Description	Kinase-Associated Endopeptidase 1

Experimental Data
Molecules/cell	1270 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	386
Molecular Weight (Da)	42,748
Isoelectric Point (pI)	7.18

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Kae1p (InterPro)
Physical Interactions	There are 34 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001D4794 \| P36132 MIPS: YKR038C NCBI: 37362674 \| 486477 \| 549609 \| 93141283 \| NP_012964.2 GenBank/EMBL/DDBJ: DAA09191.1 \| Z28263
External Classifications	EC: 3.4.24.- [Metalloendopeptidases]

Amino Acid Sequence (or in FASTA format)
1 MVNLNTIPPK NGRDYYIALG LEGSANKLGV GIVKHPLLPK HANSDLSYDC 51 EAEMLSNIRD TYVTPPGEGF LPRDTARHHR NWCIRLIKQA LAEADIKSPT 101 LDIDVICFTK GPGMGAPLHS VVIAARTCSL LWDVPLVGVN HCIGHIEMGR 151 EITKAQNPVV LYVSGGNTQV IAYSEKRYRI FGETLDIAIG NCLDRFARTL 201 KIPNEPSPGY NIEQLAKKAP HKENLVELPY TVKGMDLSMS GILASIDLLA 251 KDLFKGNKKN KILFDKTTGE QKVTVEDLCY SLQENLFAML VEITERAMAH 301 VNSNQVLIVG GVGCNVRLQE MMAQMCKDRA NGQVHATDNR FCIDNGVMIA 351 QAGLLEYRMG GIVKDFSETV VTQKFRTDEV YAAWRD*

Amino Acid Sequence (or in FASTA format)

       1  MVNLNTIPPK NGRDYYIALG LEGSANKLGV GIVKHPLLPK HANSDLSYDC
      51  EAEMLSNIRD TYVTPPGEGF LPRDTARHHR NWCIRLIKQA LAEADIKSPT
     101  LDIDVICFTK GPGMGAPLHS VVIAARTCSL LWDVPLVGVN HCIGHIEMGR
     151  EITKAQNPVV LYVSGGNTQV IAYSEKRYRI FGETLDIAIG NCLDRFARTL
     201  KIPNEPSPGY NIEQLAKKAP HKENLVELPY TVKGMDLSMS GILASIDLLA
     251  KDLFKGNKKN KILFDKTTGE QKVTVEDLCY SLQENLFAML VEITERAMAH
     301  VNSNQVLIVG GVGCNVRLQE MMAQMCKDRA NGQVHATDNR FCIDNGVMIA
     351  QAGLLEYRMG GIVKDFSETV VTQKFRTDEV YAAWRD*

external links for Kae1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Kae1p

1)	Lopreiato R, et al. (2004) Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. Biochem J 377(Pt 2):395-405
2)	Downey M, et al. (2006) A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. Cell 124(6):1155-68
3)	Kisseleva-Romanova E, et al. (2006) Yeast homolog of a cancer-testis antigen defines a new transcription complex. EMBO J 25(15):3576-85
4)	Oberto J, et al. (2009) Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Nucleic Acids Res 37(16):5343-52
5)	Srinivasan M, et al. (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81
6)	Daugeron MC, et al. (2011) Gcn4 misregulation reveals a direct role for the evolutionary conserved EKC/KEOPS in the t6A modification of tRNAs. Nucleic Acids Res 39(14):6148-60
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41