CAF4/YKR036C Protein Information

Standard Name	Caf4p 1
Systematic Name	Ykr036cp
ORF Classification	Verified
Description	WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication (1, 2, 3)
Name Description	CCR4 Associated Factor 1

Experimental Data
Molecules/cell	589 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	643
Molecular Weight (Da)	72,832
Isoelectric Point (pI)	5.13

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Caf4p (InterPro)
Physical Interactions	There are 40 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD62 \| P36130 MIPS: YKR036C NCBI: 160285789 \| 160285790 \| 330443664 \| 347595807 \| 486473 \| 549607 \| NP_012962.3 GenBank/EMBL/DDBJ: DAA09189.2 \| Z28261

Amino Acid Sequence (or in FASTA format)
1 MGSGDTRGES SLVAKPIEII LNKLPHAILA QQQFQKYITS PIYRYLSKLL 51 LFREVAWPES TKDTQKGQVG IFSFQNNYAD SATTFRILAH LDEQRYPLPN 101 GAAEKNLPSL FEGFKATVSI IQQRLLLDNV DGATNSDKEK YVQLPDINTG 151 FVNKTYSRID LTHLLEDVET NVENLSINKT LEMDELTRLD SMINELESRK 201 LKILERVKHI DSKSTNLEND VTLIKDRINF IEEYNLEADR EQSLRKQMEE 251 ERSSEASSFT QNEEAISSLC DVESKDTRLK DFYKMPHEKS HDKNRQIISE 301 TYSRNTTAFR MTIPHGEHGN SITALDFDTP WGTLCSSSYQ DRIVKVWDLN 351 HGIQVGELPG HLATVNCMQI DKKNYNMLIT GSKDATLKLW DLNLSREIYL 401 DHSPLKEKTE EIVTPCIHNF ELHKDEITAL SFDSEALVSG SRDKKIFHWD 451 LTTGKCIQQL DLIFTPTHSD IKMPARSLNN GACLLGTEAP MIGALQCYNS 501 ALATGTKDGI VRLWDLRVGK PVRLLEGHTD GITSLKFDSE KLVTGSMDNS 551 VRIWDLRTSS ILDVIAYDLP VSSLDFDGKL ITVGANEGGV NVFNMERDEH 601 WMTPEPPHSL DGDELSRRIA IVKYKDGFLI NGHNDGDINV WTL*

Amino Acid Sequence (or in FASTA format)

       1  MGSGDTRGES SLVAKPIEII LNKLPHAILA QQQFQKYITS PIYRYLSKLL
      51  LFREVAWPES TKDTQKGQVG IFSFQNNYAD SATTFRILAH LDEQRYPLPN
     101  GAAEKNLPSL FEGFKATVSI IQQRLLLDNV DGATNSDKEK YVQLPDINTG
     151  FVNKTYSRID LTHLLEDVET NVENLSINKT LEMDELTRLD SMINELESRK
     201  LKILERVKHI DSKSTNLEND VTLIKDRINF IEEYNLEADR EQSLRKQMEE
     251  ERSSEASSFT QNEEAISSLC DVESKDTRLK DFYKMPHEKS HDKNRQIISE
     301  TYSRNTTAFR MTIPHGEHGN SITALDFDTP WGTLCSSSYQ DRIVKVWDLN
     351  HGIQVGELPG HLATVNCMQI DKKNYNMLIT GSKDATLKLW DLNLSREIYL
     401  DHSPLKEKTE EIVTPCIHNF ELHKDEITAL SFDSEALVSG SRDKKIFHWD
     451  LTTGKCIQQL DLIFTPTHSD IKMPARSLNN GACLLGTEAP MIGALQCYNS
     501  ALATGTKDGI VRLWDLRVGK PVRLLEGHTD GITSLKFDSE KLVTGSMDNS
     551  VRIWDLRTSS ILDVIAYDLP VSSLDFDGKL ITVGANEGGV NVFNMERDEH
     601  WMTPEPPHSL DGDELSRRIA IVKYKDGFLI NGHNDGDINV WTL*

external links for Caf4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Caf4p

1)	Liu HY, et al. (2001) Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme. J Biol Chem 276(10):7541-8
2)	Griffin EE, et al. (2005) The WD40 protein Caf4p is a component of the mitochondrial fission machinery and recruits Dnm1p to mitochondria. J Cell Biol 170(2):237-48
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41