VPS1/YKR001C Protein Information

Standard Name	Vps1p 1, 2, 3
Systematic Name	Ykr001cp
Alias	Grd1p , Lam1p , Spo15p , Vpl1p 4 , Vpt26p 1
ORF Classification	Verified
Description	Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis (5, 6, 7, 8, 9)
Name Description	Vacuolar Protein Sorting 1, 2

Experimental Data
Molecules/cell	5960 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	704
Molecular Weight (Da)	78,736
Isoelectric Point (pI)	8.15

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vps1p (InterPro)
Physical Interactions	There are 81 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F05 \| P21576 MIPS: YKR001C NCBI: 173183 \| 3528 \| 401402 \| 4528 \| 486405 \| 6322853 \| NP_012926.1 GenBank/EMBL/DDBJ: DAA09157.1 \| M33315 \| X54316 \| X65124 \| Z28226

Amino Acid Sequence (or in FASTA format)
1 MDEHLISTIN KLQDALAPLG GGSQSPIDLP QITVVGSQSS GKSSVLENIV 51 GRDFLPRGTG IVTRRPLVLQ LINRRPKKSE HAKVNQTANE LIDLNINDDD 101 KKKDESGKHQ NEGQSEDNKE EWGEFLHLPG KKFYNFDEIR KEIVKETDKV 151 TGANSGISSV PINLRIYSPH VLTLTLVDLP GLTKVPVGDQ PPDIERQIKD 201 MLLKYISKPN AIILSVNAAN TDLANSDGLK LAREVDPEGT RTIGVLTKVD 251 LMDQGTDVID ILAGRVIPLR YGYIPVINRG QKDIEHKKTI REALENERKF 301 FENHPSYSSK AHYCGTPYLA KKLNSILLHH IRQTLPEIKA KIEATLKKYQ 351 NELINLGPET MDSASSVVLS MITDFSNEYA GILDGEAKEL SSQELSGGAR 401 ISYVFHETFK NGVDSLDPFD QIKDSDIRTI MYNSSGSAPS LFVGTEAFEV 451 LVKQQIRRFE EPSLRLVTLV FDELVRMLKQ IISQPKYSRY PALREAISNQ 501 FIQFLKDATI PTNEFVVDII KAEQTYINTA HPDLLKGSQA MVMVEEKLHP 551 RQVAVDPKTG KPLPTQPSSS KAPVMEEKSG FFGGFFSTKN KKKLAALESP 601 PPVLKATGQM TERETMETEV IKLLISSYFS IVKRTIADII PKALMLKLIV 651 KSKTDIQKVL LEKLYGKQDI EELTKENDIT IQRRKECKKM VEILRNASQI 701 VSSV*

Amino Acid Sequence (or in FASTA format)

       1  MDEHLISTIN KLQDALAPLG GGSQSPIDLP QITVVGSQSS GKSSVLENIV
      51  GRDFLPRGTG IVTRRPLVLQ LINRRPKKSE HAKVNQTANE LIDLNINDDD
     101  KKKDESGKHQ NEGQSEDNKE EWGEFLHLPG KKFYNFDEIR KEIVKETDKV
     151  TGANSGISSV PINLRIYSPH VLTLTLVDLP GLTKVPVGDQ PPDIERQIKD
     201  MLLKYISKPN AIILSVNAAN TDLANSDGLK LAREVDPEGT RTIGVLTKVD
     251  LMDQGTDVID ILAGRVIPLR YGYIPVINRG QKDIEHKKTI REALENERKF
     301  FENHPSYSSK AHYCGTPYLA KKLNSILLHH IRQTLPEIKA KIEATLKKYQ
     351  NELINLGPET MDSASSVVLS MITDFSNEYA GILDGEAKEL SSQELSGGAR
     401  ISYVFHETFK NGVDSLDPFD QIKDSDIRTI MYNSSGSAPS LFVGTEAFEV
     451  LVKQQIRRFE EPSLRLVTLV FDELVRMLKQ IISQPKYSRY PALREAISNQ
     501  FIQFLKDATI PTNEFVVDII KAEQTYINTA HPDLLKGSQA MVMVEEKLHP
     551  RQVAVDPKTG KPLPTQPSSS KAPVMEEKSG FFGGFFSTKN KKKLAALESP
     601  PPVLKATGQM TERETMETEV IKLLISSYFS IVKRTIADII PKALMLKLIV
     651  KSKTDIQKVL LEKLYGKQDI EELTKENDIT IQRRKECKKM VEILRNASQI
     701  VSSV*

external links for Vps1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Vps1p

1)	Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2)	Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3)	Bloom, K. (1992) Personal Communication, Mortimer Map Edition 11
4)	Rothman JH and Stevens TH (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
5)	Ekena K, et al. (1993) The VPS1 protein is a dynamin-like GTPase required for sorting proteins to the yeast vacuole. Ciba Found Symp 176():198-211; discussion 211-4
6)	Nothwehr SF, et al. (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
7)	Vizeacoumar FJ, et al. (2003) YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae. J Cell Biol 161(2):321-32
8)	Yu X and Cai M (2004) The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p. J Cell Sci 117(Pt 17):3839-53
9)	Rooij II, et al. (2010) A role for the dynamin-like protein Vps1 during endocytosis in yeast. J Cell Sci 123(Pt 20):3496-506
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41