FRE2/YKL220C Protein Information Help

Standard Name Fre2p
Systematic Name Ykl220cp
ORF Classification Verified
Description Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels (1, 2, 3)
Name Description Ferric REductase
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 711
Molecular Weight (Da) 80,072
Isoelectric Point (pI) 9.82

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Fre2p (InterPro)
Transmembrane Domains There are 7 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 2 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012ABF4 | P36033
MIPS: YKL220C
NCBI: 473129 | 486395 | 549758 | 6322629 | NP_012702.1
GenBank/EMBL/DDBJ: DAA08949.1 | X75950 | Z28220
External Classifications EC: 1.16.1.-
EC: 1.16.1.7 [Ferric-chelate reductase (NADH)] TC: 1.A.20.5
Amino Acid Sequence (or in FASTA format)
 
       1  MHWTSILSAI LLFCLSGARA SPAKTVIRNK VPLLVTNACT RIFQKVTWEY
      51  TSKSKRSSPV CSYEPAFQSM LYCIYETLDE KGYSNKTLEK TFSTIKKNCA
     101  SYSDALQNMT NSEFYDVLNN GTRHMTPYVK GSANLTYPVE MDTQLRKAYY
     151  HALHGFYANL DVGNIYGGII CAYFVAIMAF AGVLHCMNYT PFKTVLLKQK
     201  LVGYVRGYLT LPTIGSKHAS DFSYFRIFTG YLPTRLEGII ILGYLVLHTV
     251  FLAYGYEYDP ENIIFKSRRV QVARYVADRS GVLAFAHFPL IVLFAGRNNF
     301  LEYISGVKYT SFIMFHKWLG RMMFLDAMIH GSAYTSYTVA NKTWATSKNR
     351  LYWQFGVAAL CLAGTMVFFS FAVFRKYFYE AFLFLHIVLG AMFFYACWEH
     401  VVSLSGIEWI YTAIAIWIVD RIIRIIKASY FGFPKASLQL IGDDLIRLTV
     451  KKPARPWRAK PGQYVFVSFL HPLYFWQSHP FTVLDSVSKN GELVIILKEK
     501  KGVTRLVKKY VCRNGGKTSM RLAIEGPYGS SSPVNNYNNV LLLTGGTGLP
     551  GPIAHAIKLG KTSAAAGKQS VKLVIAVRGF DVLEAYKPEL MCLENLNVQL
     601  HIYNTMEVPS LTPSDSLDIS QQDEKADEKG TVVATTLEKS ANPLGFDGVV
     651  FHCGRPNVKE LLHEAAELSG SLSVVCCGPP IFVDKVRNET AKIVLDKSAK
     701  AIEYFEEYQC W*

external links for Fre2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS
YOGY CYC2008 (complexes) Pfam domains

Complexome TCDB

DIP YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Fre2p
1) Georgatsou E, et al.  (1997) The yeast Fre1p/Fre2p cupric reductases facilitate copper uptake and are regulated by the copper-modulated Mac1p activator. J Biol Chem 272(21):13786-92
2) Georgatsou E and Alexandraki D  (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
3) Yun CW, et al.  (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23