JEN1/YKL217W Protein Information 
| Standard Name | Jen1p 1 |
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| Systematic Name | Ykl217wp |
| ORF Classification | Verified |
| Description | Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose (2, 3, 4, 5, 6, 7, 8, 9) |
| Click on image for expanded interactive view |
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| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Jen1p (InterPro) |
| Transmembrane Domains | There are 12 total predicted transmembrane domains (TMHMM) |
| Physical Interactions | There are 4 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI000012DAAE | P36035 MIPS: YKL217W NCBI: 473133 | 486389 | 549755 | 6322632 | 780802 | NP_012705.1 GenBank/EMBL/DDBJ: DAA08952.1 | U24155 | X75951 | Z28217 |
| External Classifications | TC: 2.A.1.12 [The Sialate:H+ Symporter (SHS) Family] |
external links for Jen1p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | Organelle DB |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YPL+ |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastGFP |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | |
| Candida (CandidaDB) | Complexome | TCDB | |
| YGOB | DIP | YeastRC Structure Prediction (Seattle) | |
| YOGY | GeneMANIA |
References cited on this page View Complete Literature Guide for Jen1p
| 1) | Davis, E.S. (1994) Molecular Analysis of Cyclophilin Function in the yeast Saccharomyces cerevisiae. Ph.D. Thesis, University of Maryland |
| 2) | Haurie V, et al. (2001) The transcriptional activator Cat8p provides a major contribution to the reprogramming of carbon metabolism during the diauxic shift in Saccharomyces cerevisiae. J Biol Chem 276(1):76-85 |
| 3) | Casal M, et al. (1999) The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1. J Bacteriol 181(8):2620-3 |
| 4) | Akita O, et al. (2000) Transport of pyruvate in Saccharomyces cerevisiae and cloning of the gene encoded pyruvate permease. Biosci Biotechnol Biochem 64(5):980-4 |
| 5) | Paiva S, et al. (2002) Utilization of green fluorescent protein as a marker for studying the expression and turnover of the monocarboxylate permease Jen1p of Saccharomyces cerevisiae. Biochem J 363(Pt 3):737-44 |
| 6) | Chambers P, et al. (2004) Saccharomyces cerevisiae JEN1 promoter activity is inversely related to concentration of repressing sugar. Appl Environ Microbiol 70(1):8-17 |
| 7) | Andrade RP, et al. (2005) Multiple transcripts regulate glucose-triggered mRNA decay of the lactate transporter JEN1 from Saccharomyces cerevisiae. Biochem Biophys Res Commun 332(1):254-62 |
| 8) | Soares-Silva I, et al. (2007) The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H(+) symporter subfamily defines the function of the substrate translocation pathway. Mol Membr Biol 24(5):464-74 |
| 9) | McDermott JR, et al. (2010) Jen1p: a high affinity selenite transporter in yeast. Mol Biol Cell 21(22):3934-41 |
