URA1/YKL216W Protein Information

Standard Name	Ura1p 1
Systematic Name	Ykl216wp
ORF Classification	Verified
Description	Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid (2, 3, 4)
Name Description	URAcil requiring 2

Experimental Data
Molecules/cell	264000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	314
Molecular Weight (Da)	34,801
Isoelectric Point (pI)	5.92

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ura1p (InterPro)
Physical Interactions	There are 16 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000132CA6 \| P28272 MIPS: YKL216W NCBI: 131723 \| 171394 \| 45719878 \| 45719880 \| 45719882 \| 45719884 \| 45719886 \| 45719888 \| 45719890 \| 45719892 \| 45719894 \| 45719896 \| 45719898 \| 45719900 \| 45719902 \| 45719904 \| 45719906 \| 473134 \| 4754 \| 486387 \| 6322633 \| NP_012706.1 GenBank/EMBL/DDBJ: DAA08953.1 \| AJ585637 \| AJ585638 \| AJ585639 \| AJ585640 \| AJ585641 \| AJ585642 \| AJ585643 \| AJ585644 \| AJ585645 \| AJ585646 \| AJ585647 \| AJ585648 \| AJ585649 \| AJ585650 \| AJ585651 \| M83295 \| X59371 \| X75951 \| Z28216
External Classifications	EC: 1.3.5.2 [Dihydroorotate dehydrogenase (quinone)]

Amino Acid Sequence (or in FASTA format)
1 MTASLTTKFL NNTYENPFMN ASGVHCMTTQ ELDELANSKA GAFITKSATT 51 LEREGNPEPR YISVPLGSIN SMGLPNEGID YYLSYVLNRQ KNYPDAPAIF 101 FSVAGMSIDE NLNLLRKIQD SEFNGITELN LSCPNVPGKP QVAYDFDLTK 151 ETLEKVFAFF KKPLGVKLPP YFDFAHFDIM AKILNEFPLA YVNSINSIGN 201 GLFIDVEKES VVVKPKNGFG GIGGEYVKPT ALANVRAFYT RLRPEIKVIG 251 TGGIKSGKDA FEHLLCGASM LQIGTELQKE GVKIFERIEK ELKDIMEAKG 301 YTSIDQFRGK LNSI*

Amino Acid Sequence (or in FASTA format)

       1  MTASLTTKFL NNTYENPFMN ASGVHCMTTQ ELDELANSKA GAFITKSATT
      51  LEREGNPEPR YISVPLGSIN SMGLPNEGID YYLSYVLNRQ KNYPDAPAIF
     101  FSVAGMSIDE NLNLLRKIQD SEFNGITELN LSCPNVPGKP QVAYDFDLTK
     151  ETLEKVFAFF KKPLGVKLPP YFDFAHFDIM AKILNEFPLA YVNSINSIGN
     201  GLFIDVEKES VVVKPKNGFG GIGGEYVKPT ALANVRAFYT RLRPEIKVIG
     251  TGGIKSGKDA FEHLLCGASM LQIGTELQKE GVKIFERIEK ELKDIMEAKG
     301  YTSIDQFRGK LNSI*

external links for Ura1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ura1p

1)	Link, A. and Olson, M. (1989) Personal Communication, Mortimer Map Edition 10
2)	Guerry-Kopecko P and Wickner RB (1980) Cloning of the URA1 gene of Saccharomyces cerevisiae. J Bacteriol 143(3):1530-3
3)	Lacroute F (1968) Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol 95(3):824-32
4)	Vorisek J, et al. (2002) Enzymatic activities of Ura2 and Ura1 proteins (aspartate carbamoyltransferase and dihydro-orotate dehydrogenase) are present in both isolated membranes and cytoplasm of Saccharomyces cerevisiae. Yeast 19(5):449-57
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41