DOA1/YKL213C Protein Information Help

Standard Name Doa1p 1
Systematic Name Ykl213cp
Alias Ufd3p 2 , Zzz4p 3
ORF Classification Verified
Description WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress (2, 4, 5, 6)
Name Description Degradation Of Alpha 1
Experimental Data
Molecules/cell 6800 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 715
Molecular Weight (Da) 79,505
Isoelectric Point (pI) 4.71

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Doa1p (InterPro)
Physical Interactions There are 58 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053183 | P36037
MIPS: YKL213C
NCBI: 1086570 | 281307031 | 281307032 | 312597286 | 313103899 | 313103900 | 364505965 | 364505966 | 473137 | 486381 | 549752 | 6322636 | NP_012709.1
GenBank/EMBL/DDBJ: DAA08956.1 | U39947 | X75951 | Z28213
Amino Acid Sequence (or in FASTA format)
 
       1  MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV
      51  YTGQGFLNSV CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG
     101  NVCSLSFQDG VVISGSWDKT AKVWKEGSLV YNLQAHNASV WDAKVVSFSE
     151  NKFLTASADK TIKLWQNDKV IKTFSGIHND VVRHLAVVDD GHFISCSNDG
     201  LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED RTVRIWSKEN
     251  GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI
     301  NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI
     351  EAHQFSNSSW KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL
     401  KLPINVSDNP YTAADNFLAR YELPMSYRDQ VVQFILKNTN GISLDQPNDN
     451  ASSSAVSPSK TSVMKVLPVK QYLIMENYNP DTIFNGIVKI NSNEKTFDDE
     501  ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV RLIVKKLPYS
     551  SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF
     601  ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI
     651  NTKYGPLEEY QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS
     701  YGNVPRFKDI FDDLS*

external links for Doa1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Doa1p
1) Hochstrasser M and Varshavsky A  (1990) In vivo degradation of a transcriptional regulator: the yeast alpha 2 repressor. Cell 61(4):697-708
2) Johnson ES, et al.  (1995) A proteolytic pathway that recognizes ubiquitin as a degradation signal. J Biol Chem 270(29):17442-56
3) Keil RL, et al.  (1996) Molecular genetic analysis of volatile-anesthetic action. Mol Cell Biol 16(7):3446-53
4) Ghislain M, et al.  (1996) Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae. EMBO J 15(18):4884-99
5) Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41