SAC1/YKL212W Protein Information 
| Standard Name | Sac1p 1 |
|---|---|
| Systematic Name | Ykl212wp |
| Alias | Rsd1p 2 |
| ORF Classification | Verified |
| Description | Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism (3, 4, 5, 6, 7, 8) |
| Name Description | Suppressor of ACtin 9 |
| Experimental Data | |
|---|---|
| Molecules/cell | 48000 10 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Sac1p (InterPro) |
| Transmembrane Domains | There are 2 total predicted transmembrane domains (TMHMM) |
| Physical Interactions | There are 75 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI00001351E8 | P32368 MIPS: YKL212W NCBI: 1086569 | 295789484 | 417726 | 4388552 | 473138 | 486379 | 6322637 | NP_012710.1 GenBank/EMBL/DDBJ: DAA08957.1 | U39947 | X51672 | X75951 | Z28212 |
| External Classifications | EC: 3.1.3.- [Phosphoric Monoester Hydrolases] |
external links for Sac1p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | Organelle DB |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YPL+ |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastGFP |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | YeastRC Public Image Repository |
| Candida (CandidaDB) | Complexome | YeastRC Structure Prediction (Seattle) | |
| YGOB | DIP | ||
| YOGY | GeneMANIA | ||
| YeastRC Two-Hybrid (Seattle) |
References cited on this page View Complete Literature Guide for Sac1p
| 1) | Drubin, D. and Botstein, D. (1989) Personal Communication, Mortimer Map Edition 10 |
| 2) | Cleves AE, et al. (1989) Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function. J Cell Biol 109(6 Pt 1):2939-50 |
| 3) | Whitters EA, et al. (1993) SAC1p is an integral membrane protein that influences the cellular requirement for phospholipid transfer protein function and inositol in yeast. J Cell Biol 122(1):79-94 |
| 4) | Guo S, et al. (1999) SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem 274(19):12990-5 |
| 5) | Hughes WE, et al. (2000) SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases. J Biol Chem 275(2):801-8 |
| 6) | Schorr M, et al. (2001) The phosphoinositide phosphatase Sac1p controls trafficking of the yeast Chs3p chitin synthase. Curr Biol 11(18):1421-6 |
| 7) | Brice SE, et al. (2009) Modulation of Sphingolipid Metabolism by the Phosphatidylinositol-4-phosphate Phosphatase Sac1p through Regulation of Phosphatidylinositol in Saccharomyces cerevisiae. J Biol Chem 284(12):7588-96 |
| 8) | Wood CS, et al. (2012) Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase. Mol Biol Cell 23(13):2527-36 |
| 9) | Novick P, et al. (1989) Suppressors of yeast actin mutations. Genetics 121(4):659-74 |
| 10) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
