TRP3/YKL211C Protein Information

Standard Name	Trp3p 1
Systematic Name	Ykl211cp
ORF Classification	Verified
Description	Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p (2, 3, 4, 5)
Name Description	TRyPtophan requiring

Experimental Data
Molecules/cell	13400 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	484
Molecular Weight (Da)	53,489
Isoelectric Point (pI)	6.9

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Trp3p (InterPro)
Physical Interactions	There are 35 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052EA8 \| P00937 MIPS: YKL211C NCBI: 171126 \| 173045 \| 473139 \| 486377 \| 549127 \| 6322638 \| NP_012711.1 GenBank/EMBL/DDBJ: DAA08958.1 \| K01386 \| M36300 \| X75951 \| Z28211
External Classifications	EC: 4.1.1.48 [Indole-3-glycerol-phosphate synthase] EC: 4.1.3.27 [Anthranilate synthase]

Amino Acid Sequence (or in FASTA format)
1 MSVHAATNPI NKHVVLIDNY DSFTWNVYEY LCQEGAKVSV YRNDAITVPE 51 IAALNPDTLL ISPGPGHPKT DSGISRDCIR YFTGKIPVFG ICMGQQCMFD 101 VFGGEVAYAG EIVHGKTSPI SHDNCGIFKN VPQGIAVTRY HSLAGTESSL 151 PSCLKVTAST ENGIIMGVRH KKYTVEGVQF HPESILTEEG HLMIRNILNV 201 SGGTWEENKS SPSNSILDRI YARRKIDVNE QSKIPGFTFQ DLQSNYDLGL 251 APPLQDFYTV LSSSHKRAVV LAEVKRASPS KGPICLKAVA AEQALKYAEA 301 GASAISVLTE PHWFHGSLQD LVNVRKILDL KFPPKERPCV LRKEFIFSKY 351 QILEARLAGA DTVLLIVKML SQPLLKELYS YSKDLNMEPL VEVNSKEELQ 401 RALEIGAKVV GVNNRDLHSF NVDLNTTSNL VESIPKDVLL IALSGITTRD 451 DAEKYKKEGV HGFLVGEALM KSTDVKKFIH ELCE*

Amino Acid Sequence (or in FASTA format)

       1  MSVHAATNPI NKHVVLIDNY DSFTWNVYEY LCQEGAKVSV YRNDAITVPE
      51  IAALNPDTLL ISPGPGHPKT DSGISRDCIR YFTGKIPVFG ICMGQQCMFD
     101  VFGGEVAYAG EIVHGKTSPI SHDNCGIFKN VPQGIAVTRY HSLAGTESSL
     151  PSCLKVTAST ENGIIMGVRH KKYTVEGVQF HPESILTEEG HLMIRNILNV
     201  SGGTWEENKS SPSNSILDRI YARRKIDVNE QSKIPGFTFQ DLQSNYDLGL
     251  APPLQDFYTV LSSSHKRAVV LAEVKRASPS KGPICLKAVA AEQALKYAEA
     301  GASAISVLTE PHWFHGSLQD LVNVRKILDL KFPPKERPCV LRKEFIFSKY
     351  QILEARLAGA DTVLLIVKML SQPLLKELYS YSKDLNMEPL VEVNSKEELQ
     401  RALEIGAKVV GVNNRDLHSF NVDLNTTSNL VESIPKDVLL IALSGITTRD
     451  DAEKYKKEGV HGFLVGEALM KSTDVKKFIH ELCE*

external links for Trp3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Trp3p

1)	Link, A. and Olson, M. (1989) Personal Communication, Mortimer Map Edition 10
2)	Paluh JL and Zalkin H (1983) Isolation of Saccharomyces cerevisiae TRP3. J Bacteriol 153(1):345-9
3)	Zalkin H, et al. (1984) Nucleotide sequence of Saccharomyces cerevisiae genes TRP2 and TRP3 encoding bifunctional anthranilate synthase: indole-3-glycerol phosphate synthase. J Biol Chem 259(6):3985-92
4)	Prantl F, et al. (1985) Purification and characterization of the indole-3-glycerolphosphate synthase/anthranilate synthase complex of Saccharomyces cerevisiae. Eur J Biochem 146(1):95-100
5)	Fantes PA, et al. (1976) Free tryptophan pool and tryptophan biosynthetic enzymes in Saccharomyces cerevisiae. Arch Microbiol 107(2):207-14
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41