STE6/YKL209C Protein Information

Standard Name	Ste6p
Systematic Name	Ykl209cp
ORF Classification	Verified
Description	Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells (1, 2, 3, 4, 5)
Name Description	STErile

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,290
Molecular Weight (Da)	144,764
Isoelectric Point (pI)	7.91

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ste6p (InterPro)
Transmembrane Domains	There are 10 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 18 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001360DD \| P12866 MIPS: YKL209C NCBI: 134967 \| 172758 \| 4564 \| 486373 \| 553144 \| 6322640 \| NP_012713.1 GenBank/EMBL/DDBJ: DAA08960.1 \| M12842 \| M26376 \| X15428 \| Z28209
External Classifications	TC: 3.A.1.206 [The alpha Factor Sex Pheromone Exporter (STE) Family]

Amino Acid Sequence (or in FASTA format)
1 MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR 51 VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG 101 ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE 151 ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV 201 YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF 251 IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC 301 IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL 351 DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST 401 LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR 451 KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG 501 TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH 551 WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST 601 WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE 651 TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI 701 IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD 751 GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM 801 EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS 851 TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL 901 ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS 951 MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS 1001 QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP 1051 IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL 1101 TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY 1151 GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI 1201 ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM 1251 MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS *

Amino Acid Sequence (or in FASTA format)

       1  MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR
      51  VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG
     101  ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE
     151  ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV
     201  YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF
     251  IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC
     301  IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL
     351  DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST
     401  LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR
     451  KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG
     501  TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH
     551  WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST
     601  WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE
     651  TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI
     701  IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD
     751  GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM
     801  EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS
     851  TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL
     901  ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS
     951  MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS
    1001  QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP
    1051  IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL
    1101  TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY
    1151  GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI
    1201  ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM
    1251  MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS *

external links for Ste6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	TCDB
YOGY	DIP	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Ste6p

1)	McGrath JP and Varshavsky A (1989) The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein. Nature 340(6232):400-4
2)	Michaelis S (1993) STE6, the yeast a-factor transporter. Semin Cell Biol 4(1):17-27
3)	Kuchler K, et al. (1989) Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells. EMBO J 8(13):3973-84
4)	Loayza D and Michaelis S (1998) Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the a-factor transporter in Saccharomyces cerevisiae. Mol Cell Biol 18(2):779-89
5)	Ketchum CJ, et al. (2001) The yeast a-factor transporter Ste6p, a member of the ABC superfamily, couples ATP hydrolysis to pheromone export. J Biol Chem 276(31):29007-11