STE6/YKL209C Protein Information Help

Standard Name Ste6p
Systematic Name Ykl209cp
ORF Classification Verified
Description Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells (1, 2, 3, 4, 5)
Name Description STErile
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,290
Molecular Weight (Da) 144,764
Isoelectric Point (pI) 7.91

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ste6p (InterPro)
Transmembrane Domains There are 10 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 26 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001360DD | P12866
MIPS: YKL209C
NCBI: 134967 | 172758 | 4564 | 486373 | 553144 | 6322640 | NP_012713.1 | NM_001179774.1
GenBank/EMBL/DDBJ: AAA35116.1 | AAA35117.1 | CAA33467.1 | CAA82054.1 | DAA08960.1 | M12842 | M26376 | X15428 | Z28209
External Classifications EC: 3.6.3.48 [Alpha-factor-transporting ATPase] TC: 3.A.1.206 [The alpha Factor Sex Pheromone Exporter (STE) Family]
Amino Acid Sequence (or in FASTA format)
       1  MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR
      51  VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG
     101  ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE
     151  ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV
     201  YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF
     251  IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC
     301  IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL
     351  DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST
     401  LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR
     451  KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG
     501  TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH
     551  WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST
     601  WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE
     651  TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI
     701  IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD
     751  GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM
     801  EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS
     851  TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL
     901  ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS
     951  MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS
    1001  QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP
    1051  IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL
    1101  TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY
    1151  GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI
    1201  ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM
    1251  MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS *         

external links for Ste6p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) CYC2008 (complexes) MIPS YeastGFP
YGOB Complexome Pfam domains YeastRC Public Image Repository
YOGY DIP TCDB

GeneMANIA YeastRC Structure Prediction (Seattle)

IMP

References cited on this page View Complete Literature Guide for Ste6p
1) McGrath JP and Varshavsky A  (1989) The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein. Nature 340(6232):400-4
2) Michaelis S  (1993) STE6, the yeast a-factor transporter. Semin Cell Biol 4(1):17-27
3) Kuchler K, et al.  (1989) Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells. EMBO J 8(13):3973-84
4) Loayza D and Michaelis S  (1998) Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the a-factor transporter in Saccharomyces cerevisiae. Mol Cell Biol 18(2):779-89
5) Ketchum CJ, et al.  (2001) The yeast a-factor transporter Ste6p, a member of the ABC superfamily, couples ATP hydrolysis to pheromone export. J Biol Chem 276(31):29007-11