LOS1/YKL205W Protein Information Help

Standard Name Los1p
Systematic Name Ykl205wp
ORF Classification Verified
Description Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm (1, 2, 3, 4)
Name Description Loss Of Suppression 5
Experimental Data
Molecules/cell 3490 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,100
Molecular Weight (Da) 126,818
Isoelectric Point (pI) 4.86

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Los1p (InterPro)
Physical Interactions There are 23 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012E806 | P33418
MIPS: YKL205W
NCBI: 349395 | 462544 | 486365 | 6322644 | NP_012717.1
GenBank/EMBL/DDBJ: DAA08964.1 | L13941 | Z28205
Amino Acid Sequence (or in FASTA format)
 
       1  MLERIQQLVN AVNDPRSDVA TKRQAIELLN GIKSSENALE IFISLVINEN
      51  SNDLLKFYGL STLIELMTEG VNANPNGLNL VKFEITKWLK FQVLGNKQTK
     101  LPDFLMNKIS EVLTTLFMLM YSDCNGNQWN SFFDDLMSLF QVDSAISNTS
     151  PSTDGNILLG LEFFNKLCLM INSEIADQSF IRSKESQLKN NNIKDWMRDN
     201  DIMKLSNVWF QCLKLDEQIV SQCPGLINST LDCIGSFISW IDINLIIDAN
     251  NYYLQLIYKF LNLKETKISC YNCILAIISK KMKPMDKLAF LNMINLTNEL
     301  TYYHQAISMN PQIITFDNLE VWESLTKLIT SFGIEFTIII EQVNDDQKLD
     351  TLYKQSVISN VDSILLEKII PILLEFMNNE FDSITAKTFP FWSNYLAFLK
     401  KYKASSPNFV PLHKDFLDNF QQICFKRMKF SDDEVTQDDF EEFNETVRFK
     451  LKNFQEIIVV IDPSLFLNNI SQEISANLMN CKNESWQIFE LTIYQIFNLS
     501  ECTKNNYFGL NKNEIMTSQP SLTLVRFLNE LLMMKDFLLA IDNEQIQILF
     551  MELIVKNYNF IFSTSANTAN ATDDDEKYLL ILNIFMSSFA MFNKRENVRL
     601  RSWYLFTRFL KLTRINLKKI LFANKNLVNE ITNKISPLLH IKVTSINAQG
     651  TDDNDTIFDN QLYIFEGIGF IITLNNSSQE LTAATANTPI DYDILDQILT
     701  PLFTQLEGCI TQGASPVVIL ECHHILMAIG TLARGLHIGL VPENQVNNMV
     751  VNKKLINDSL IHKFSNIAEV ILVTFSFFNK FENIRDASRF TFARLIPILS
     801  NKILPFINKL IELILSSTDL KSWEMIDFLG FLSQLIHMFH TDTDCYQLFN
     851  QLLTPLINKV HSIIEEIDEQ HDQQSSSNKP IDTAVTATSV NKNIVVTDSY
     901  RDKILLKKAY CTFLQSFTNN SVTSILLSDI NRAILPVILN DLVTYTPQEI
     951  QETSMMKVSL NVLCNFIKCF GNGTCLDNDD INKDPNLKID GLNEYFIMKC
    1001  VPIIFEIPFN PIYKFNIKEG NFKTMAYDLA RLLRELFIVS SNPTTNENEC
    1051  VKYLTQIYLP QIQLPQELTI QLVNMLTTMG QKQFEKWFVD NFISVLKQGQ
    1101  *

external links for Los1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Los1p
1) Hurt DJ, et al.  (1987) Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing. Mol Cell Biol 7(3):1208-16
2) Hellmuth K, et al.  (1998) Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol Cell Biol 18(11):6374-86
3) Shen WC, et al.  (1993) The Saccharomyces cerevisiae LOS1 gene involved in pre-tRNA splicing encodes a nuclear protein that behaves as a component of the nuclear matrix. J Biol Chem 268(26):19436-44
4) Murthi A, et al.  (2010) Regulation of tRNA Bidirectional Nuclear-Cytoplasmic Trafficking in Saccharomyces cerevisiae. Mol Biol Cell 21(4):639-49
5) Hopper AK, et al.  (1980) Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs. Cell 19(3):741-51
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41