NNK1/YKL171W Protein Information Help

Standard Name Nnk1p 1
Systematic Name Ykl171wp
ORF Classification Verified
Description Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm (1, 2, 3, 4, 5)
Name Description Nitrogen Network Kinase 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 928
Molecular Weight (Da) 103,955
Isoelectric Point (pI) 9.26

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Nnk1p (InterPro)
Physical Interactions There are 70 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DEC4 | P36003
MIPS: YKL171W
NCBI: 407511 | 486300 | 547785 | 6322677 | NP_012750.1
GenBank/EMBL/DDBJ: DAA08995.1 | Z26878 | Z28171
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MFTSQRQLRQ NGSPMSSSRS SQHSSGTASP ISDSPASNRS YGRDLRGLMG
      51  IDIPANEPAF NRANSSDTIY FRPKKIYKME HEHPSRSTLV QLQTRSQPDD
     101  VASSQVNPEG GTDDLELGDP CGNQSLYTIG AEYVPDLDFT KLVNEWQKST
     151  EDLYEFRSSA TPQVQIKDSG KGNYELWSSP DAILTQNKLR RDSFSQENSD
     201  SLSPEDSILS RNLHSKVKPI PLPRNSQQIF TPLSNLEAER RSSYTTSSNN
     251  NSITQNNKFS FAKLKYSLPT QSSAVPASFD SNASSLNFLP TTTLSTLSEL
     301  QISPNDMMDL IQKLPRNFLN LPYTQRKKVI IEHAPSHDYK AMMSLVKKFM
     351  LTSSRSNFSL AGFANNASVS QATANDDNIN SRNTPNNSND TYVNTRPLQR
     401  SRHGSIASQF LSSFSPSMTS IAKMNSNPLS GSAGGSARPD DKGMEILGHR
     451  LGKIIGFGAW GIIRECFDIE TGVGRVIKIV KFKGHQNIKK HVLREVAIWR
     501  TLKHNRILPL LDWKLDDNYA MYCLTERIND GTLYDLVISW DEFKRSKIPF
     551  AERCRLTIFL SLQLLSALKY MHSKTIVHGD IKLENCLLQK EGKKSDWKVF
     601  LCDFGMSCHF DEKHVYRNDT FDENLSSGNS HRKRKSIEQT NLIKYPTTNF
     651  LPDDRTNDFD ASENLKYQFE NRKHQPFTPK GMVSSSSHSL KHLNQPSSSS
     701  SSNLFHKPAS QPQPQHRSPF HGRHKTTDFS NLEPEPSKYI GSLPYASPEL
     751  LRYSDARRSK SVEMHIYDSP DSSQSEISAA SSSSSNLSSL SSSTKASAVT
     801  NSGVTTSSPS GSSTDFPCIV SPLGPASDIW ALGVMLYTML VGKLPFNHEF
     851  EPRLRSLIKV GEFDRFSLAQ VCKFDRKKNE GTIGQGLYDT VIGCLTIDLD
     901  KRWKLKRIEE VLQNEMNLSE AIHDNNGS*

external links for Nnk1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Nnk1p
1) Breitkreutz A, et al.  (2010) A global protein kinase and phosphatase interaction network in yeast. Science 328(5981):1043-6
2) Hunter T and Plowman GD  (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22(1):18-22
3) Cagney G, et al.  (2001) Two-hybrid analysis of the Saccharomyces cerevisiae 26S proteasome. Physiol Genomics 7(1):27-34
4) Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19
5) Miranda-Saavedra D and Barton GJ  (2007) Classification and functional annotation of eukaryotic protein kinases. Proteins 68(4):893-914