TPK3/YKL166C Protein Information

Standard Name	Tpk3p
Systematic Name	Ykl166cp
ORF Classification	Verified
Description	cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Takashi's Protein Kinase 6

Experimental Data
Molecules/cell	1590 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	398
Molecular Weight (Da)	45,977
Isoelectric Point (pI)	6.31

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tpk3p (InterPro)
Physical Interactions	There are 126 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E95 \| P05986 MIPS: YKL166C NCBI: 171979 \| 173013 \| 407516 \| 486291 \| 547757 \| 6322682 \| NP_012755.1 \| NM_001179732.1 GenBank/EMBL/DDBJ: DAA08999.1 \| M17074 \| M81697 \| Z26878 \| Z28166
External Classifications	EC: 2.7.11.11 [cAMP-dependent protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI 51 NGRNSGKLKE EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG 101 RVHLIRSNHN GRFYALKTLK KHTIVKLKQV EHTNDERRML SIVSHPFIIR 151 MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS QRFPNPVAKF YAAEVCLALE 201 YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT YTLCGTPDYI 251 APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL 301 KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL 351 LARYIETPYE PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF*

Amino Acid Sequence (or in FASTA format)

       1  MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI
      51  NGRNSGKLKE EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG
     101  RVHLIRSNHN GRFYALKTLK KHTIVKLKQV EHTNDERRML SIVSHPFIIR
     151  MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS QRFPNPVAKF YAAEVCLALE
     201  YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT YTLCGTPDYI
     251  APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL
     301  KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL
     351  LARYIETPYE PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF*

external links for Tpk3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tpk3p

1)	Toda T, et al. (1987) Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell 50(2):277-87
2)	Ordiz I, et al. (1996) Glucose-induced inactivation of isocitrate lyase in Saccharomyces cerevisiae is mediated by the cAMP-dependent protein kinase catalytic subunits Tpk1 and Tpk2. FEBS Lett 385(1-2):43-6
3)	Toda T, et al. (1987) Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol 7(4):1371-7
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Tudisca V, et al. (2010) Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions. Eur J Cell Biol 89(4):339-348
6)	Thorner J (2005) personal communication
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41