MCD4/YKL165C Protein Information Help

Standard Name Mcd4p 1
Systematic Name Ykl165cp
Alias Zrg16p 2 , Ssu21p 3 , Fsr2p 4
ORF Classification Verified
Description Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes (5)
Name Description Morphogenesis Checkpoint Dependent 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 919
Molecular Weight (Da) 105,692
Isoelectric Point (pI) 6.71

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mcd4p (InterPro)
Transmembrane Domains There are 14 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 8 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B749 | P36051
MIPS: YKL165C
NCBI: 407483 | 486289 | 549734 | 6322683 | NP_012756.1
GenBank/EMBL/DDBJ: DAA09000.1 | Z26877 | Z28165
External Classifications EC: 2.-.-.- [Transferases]
Amino Acid Sequence (or in FASTA format)
 
       1  MWNKTRTTLL AVGVLFHLFY LWSIFDIYFI SPLVHGMSPY QSTPTPPAKR
      51  LFLIVGDGLR ADTTFDKVTH PVSGKTEFLA PFIRSLVMNN ATYGISHTRM
     101  PTESRPGHVA MIAGFYEDVS AVTKGWKSNP VNFDSFFNQS THTYSFGSPD
     151  ILPMFKDGAS DPNKVDTWMY DHTFEDFTQS SIELDAFVFR HLDQLFHNST
     201  LNSTLDYEIR QDGNVFFLHL LGCDTAGHSY RPYSAEYYDN VKYIDDQIPI
     251  LIDKVNKFFA DDKTAFIFTA DHGMSAFGSH GDGHPNNTRT PLVAWGAGLN
     301  KPVHNPFPVS DNYTENWELS SIKRNDVKQA DIASLMSYLI GVNYPKNSVG
     351  ELPIAYIDGK ESDKLAALYN NARSILEQYL VKQDEVIDSQ FFYKEYFKFV
     401  EKSHSHYLEE IETLIQRISE GENYLEQEAI TLTEELMQIT LEGLHYLTTY
     451  NWRFIRTIVT FGFVGWIFFS FIIFLKSFIL ENVIDDQKAS PLSHAVFGSI
     501  GILLNWILFY QHSPFNFYMY LLFPLYFWSY IFTNRSVLRS GIKEFFKGTS
     551  PWKRVLITIS IISVYEGIVY GFFHRWTFTL ITNILAFYPF ICGVRELSVN
     601  ILWIITSVLL STFTLFDAVK IEDLNQIHLA GLLIILSAFY ALYKIHSRIN
     651  SYTRAIFAIQ ISLVAAMLAV THRSVISLQL RQGLPRESQV AGWIIFFVSL
     701  FVMPILHYRK PNNDYKVRLL IIYLTFAPSF IILTISFESL FYFLFTSYMV
     751  QWIEIENKIK EMKTQKDENW LQVLRVSVIG FFLLQVAFFG TGNVASISSF
     801  SLESVCRLLP IFDPFLMGAL LMLKLIIPYG LLSTCLGILN LKLNFKDYTI
     851  SSLIISMSDI LSLNFFYLLR TEGSWLDIGI TISNYCLAIL SSLFMLILEV
     901  LGHVLLKNVI IQDKTKKTQ*

external links for Mcd4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Mcd4p
1) Mondesert G, et al.  (1997) Identification of genes controlling growth polarity in the budding yeast Saccharomyces cerevisiae: a possible role of N-glycosylation and involvement of the exocyst complex. Genetics 147(2):421-34
2) Yuan DS  (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3) Packeiser AN, et al.  (1999) A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast. Yeast 15(14):1485-501
4) Matsumoto K, et al.  (1986) Fluphenazine-resistant Saccharomyces cerevisiae mutants defective in the cell division cycle. J Bacteriol 168(3):1352-7
5) Gaynor EC, et al.  (1999) MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast. Mol Biol Cell 10(3):627-48