PIR1/YKL164C Protein Information Help

Standard Name Pir1p 1
Systematic Name Ykl164cp
Alias Ccw6p 2
ORF Classification Verified
Description O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8)
Name Description Protein containing Internal Repeats 1
Experimental Data
Molecules/cell 1170 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 341
Molecular Weight (Da) 34,621
Isoelectric Point (pI) 6.5

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pir1p (InterPro)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 10 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000004AD15 | Q03178
MIPS: YKL164C
NCBI: 218457 | 407484 | 417492 | 486287 | 6322684 | NP_012757.1
GenBank/EMBL/DDBJ: DAA09001.1 | D13740 | Z26877 | Z28164
Amino Acid Sequence (or in FASTA format)
 
       1  MQYKKSLVAS ALVATSLAAY APKDPWSTLT PSATYKGGIT DYSSTFGIAV
      51  EPIATTASSK AKRAAAISQI GDGQIQATTK TTAAAVSQIG DGQIQATTKT
     101  KAAAVSQIGD GQIQATTKTT SAKTTAAAVS QIGDGQIQAT TKTKAAAVSQ
     151  IGDGQIQATT KTTAAAVSQI GDGQIQATTK TTAAAVSQIG DGQIQATTNT
     201  TVAPVSQITD GQIQATTLTS ATIIPSPAPA PITNGTDPVT AETCKSSGTL
     251  EMNLKGGILT DGKGRIGSIV ANRQFQFDGP PPQAGAIYAA GWSITPEGNL
     301  AIGDQDTFYQ CLSGNFYNLY DEHIGTQCNA VHLQAIDLLN C*

external links for Pir1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pir1p
1) Toh-e A, et al.  (1993) Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock. Yeast 9(5):481-94
2) Mrsa V, et al.  (1997) Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae. Yeast 13(12):1145-54
3) Mrsa V and Tanner W  (1999) Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall. Yeast 15(10A):813-20
4) Kapteyn JC, et al.  (1999) The contribution of the O-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and beta 1,6-glucan-deficient mutants. Mol Microbiol 31(6):1835-44
5) Yun DJ, et al.  (1997) Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein. Proc Natl Acad Sci U S A 94(13):7082-7
6) Vongsamphanh R, et al.  (2001) Pir1p mediates translocation of the yeast Apn1p endonuclease into the mitochondria to maintain genomic stability. Mol Cell Biol 21(5):1647-55
7) Doolin MT, et al.  (2001) Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p. Mol Microbiol 40(2):422-32
8) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41