MCR1/YKL150W Protein Information

Standard Name	Mcr1p 1, 2
Systematic Name	Ykl150wp
ORF Classification	Verified
Description	Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis (1, 3)
Name Description	Mitochondrial NADH-Cytochrome b5 Reductase 1

Experimental Data
Molecules/cell	1920 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	302
Molecular Weight (Da)	34,138
Isoelectric Point (pI)	9.33

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mcr1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F93A \| P36060 MIPS: YKL150W NCBI: 124263198 \| 1490392 \| 407497 \| 486258 \| 549725 \| 6322699 \| NP_012772.1 \| NM_001179716.1 GenBank/EMBL/DDBJ: DAA09013.1 \| EF123132 \| X81474 \| Z26877 \| Z28150
External Classifications	EC: 1.6.2.2 [Cytochrome-b5 reductase]

Amino Acid Sequence (or in FASTA format)
1 MFSRLSRSHS KALPIALGTV AIAAATAFYF ANRNQHSFVF NESNKVFKGD 51 DKWIDLPISK IEEESHDTRR FTFKLPTEDS EMGLVLASAL FAKFVTPKGS 101 NVVRPYTPVS DLSQKGHFQL VVKHYEGGKM TSHLFGLKPN DTVSFKGPIM 151 KWKWQPNQFK SITLLGAGTG INPLYQLAHH IVENPNDKTK VNLLYGNKTP 201 QDILLRKELD ALKEKYPDKF NVTYFVDDKQ DDQDFDGEIS FISKDFIQEH 251 VPGPKESTHL FVCGPPPFMN AYSGEKKSPK DQGELIGILN NLGYSKDQVF 301 KF*

Amino Acid Sequence (or in FASTA format)

       1  MFSRLSRSHS KALPIALGTV AIAAATAFYF ANRNQHSFVF NESNKVFKGD
      51  DKWIDLPISK IEEESHDTRR FTFKLPTEDS EMGLVLASAL FAKFVTPKGS
     101  NVVRPYTPVS DLSQKGHFQL VVKHYEGGKM TSHLFGLKPN DTVSFKGPIM
     151  KWKWQPNQFK SITLLGAGTG INPLYQLAHH IVENPNDKTK VNLLYGNKTP
     201  QDILLRKELD ALKEKYPDKF NVTYFVDDKQ DDQDFDGEIS FISKDFIQEH
     251  VPGPKESTHL FVCGPPPFMN AYSGEKKSPK DQGELIGILN NLGYSKDQVF
     301  KF*

external links for Mcr1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Mcr1p

1)	Hahne K, et al. (1994) Incomplete arrest in the outer membrane sorts NADH-cytochrome b5 reductase to two different submitochondrial compartments. Cell 79(5):829-39
2)	Vandenbol M and Fairhead C (2000) Mass-murder deletion of 19 ORFs from Saccharomyces cerevisiae chromosome XI. Gene 247(1-2):45-52
3)	Lamb DC, et al. (1999) Biodiversity of the P450 catalytic cycle: yeast cytochrome b5/NADH cytochrome b5 reductase complex efficiently drives the entire sterol 14-demethylation (CYP51) reaction. FEBS Lett 462(3):283-8
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41